AB228569

TMRM Assay Kit (Mitochondrial Membrane Potential)

Name: TMRM 분석 키트 (Mitochondrial Membrane Potential) (ab228569) | Abcam
Brand: Abcam Limited
SKU: AB228569
Rating: 5 (1 reviews)

TMRM 분석 키트 (Mitochondrial Membrane Potential)

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(18 제품이 사용된 논문 )

TMRM Assay Kit (Mitochondrial Membrane Potential) ab228569 is a complete kit for TMRM staining of cell cultures. Readout by flow cytometry, fluorescence plate reader, or fluorescence microscopy (Ex/Em 548/573 nm). Contains TMRM reagent, buffer and control.

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Immunocytochemistry/ Immunofluorescence - TMRM Assay Kit (Mitochondrial Membrane Potential) (AB228569)

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Supplier Data

Immunocytochemistry/ Immunofluorescence - TMRM Assay Kit (Mitochondrial Membrane Potential) (AB228569)

Human fibroblast cells stained using ab228569 TMRM Assay Kit (Mitochondrial Membrane Potential) (200 nM TMRM).

주요 정보

검출 방식

Fluorescent

샘플 타입

Suspension cells, Adherent cells

분석 유형

Cell-based

분석 플랫폼

Microplate reader, Fluor. microscope, Flow cyt.

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제품 세부 정보

TMRM Assay Kit (Mitochondrial Membrane Potential) ab228569 is designed to quantify changes in mitochondrial membrane potential in living cells using flow cytometry, fluorescence plate reader, or fluorescence microscopy. It uses the red-fluorescent probe TMRM (tetramethylrhodamine, methyl ester) that localizes in mitochondria and detects mitochondrial membrane depolarization.

Related assay kits
TMRE is the ethyl ester of tetramethylrhodamine and is a close equivalent of TMRM. TMRE is used about 3 times more than TMRM for mitochondrial membrane potential assays.
TMRE-Mitochondrial Membrane Potential Assay Kit ab113852 is our most popular assay kit for mitochondrial membrane potential analysis.

TMRM staining is used to monitor mitochondrial function. When accumulated in a negatively charge polarized mitochondria, TMRM fluoresces orange. When mitochondrial membrane potential collapses in apoptotic or metabolically stressed cells, the TMRM reagent is dispersed throughout the cell cytosol and fluorescence levels drop dramatically. This kit provides enough reagents to analyze 5 x 96 wells (500 samples).

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab228569-1Kit", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"5X Live Cell Imaging Buffer", "number":"AB228569-CMP01", "productcode":"" }, { "size":"1 x 25 µL", "name":"FCCP", "number":"AB228569-CMP02", "productcode":"" }, { "size":"1 x 100 µL", "name":"TMRM Reagent", "number":"AB228569-CMP03", "productcode":"" } ] } } }

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

Multi

적절한 장기 보관 조건

Multi

보관 정보

Please refer to protocols

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제품 프로토콜

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타겟 정보

See full target information Tetramethylrhodamine methyl ester perchlorate

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제품이 사용된 논문 (18)

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TMRM Assay Kit (Mitochondrial Membrane Potential)

TMRM 분석 키트 (Mitochondrial Membrane Potential)

Immunocytochemistry/ Immunofluorescence - TMRM Assay Kit (Mitochondrial Membrane Potential) (AB228569)

주요 정보

검출 방식

샘플 타입

분석 유형

분석 플랫폼

대체품

Biochemicals

보완 제품

Assay Kits

Assay Kits

Assay Kits

Assay Kits

제품 세부 정보

제품 구성

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (18)

Product promise