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AB141071

10-Hydroxycamptothecin, DNA topoisomerase I inhibitor

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MW 364.4 Da, Purity >99%. DNA topoisomerase I inhibitor (IC50 = 106 nM). Cytotoxic antitumour compound, more potent than camptothecin (ab120115). In vitro and in vivo activity against a broad spectrum of cancers.
2 이미지
Immunocytochemistry/ Immunofluorescence - 10-Hydroxycamptothecin, DNA topoisomerase I inhibitor (AB141071)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - 10-Hydroxycamptothecin, DNA topoisomerase I inhibitor (AB141071)

ab2893 staining γH2A.X in HeLa cells treated with 10-Hydroxycamptothecin (ab141071), by ICC/IF. Increase of γH2A.X nuclear expression correlates with increased concentration of 10-Hydroxycamptothecin, as described in literature.
The cells were incubated at 37°C for 6 hours in media containing different concentrations of ab141071 (10-Hydroxycamptothecin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2893 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight® 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Chemical Structure - 10-Hydroxycamptothecin, DNA topoisomerase I inhibitor (AB141071)
  • Chemical Structure

Lab

Chemical Structure - 10-Hydroxycamptothecin, DNA topoisomerase I inhibitor (AB141071)

2D chemical structure image of ab141071, 10-Hydroxycamptothecin, DNA topoisomerase I inhibitor

주요 정보

CAS 번호

64439-81-2

Purity

>99%

제형

Solid

form

Molecular weight

364.4 Da

Molecular formula

C<sub>2</sub><sub>0</sub>H<sub>1</sub><sub>6</sub>N<sub>2</sub>O<sub>5</sub>

PubChem

4330531

Nature

Synthetic

Solubility

Soluble in DMSO to 50 mM

생화학적 명칭

(+/-)-10-Hydroxycamptothecin

생물학적 정보

DNA topoisomerase I inhibitor (IC50 = 106 nM). Cytotoxic antitumour compound, more potent than camptothecin (ab120115). In vitro and in vivo activity against a broad spectrum of cancers.

Canonical SMILES

CCC1(C2=C(COC1=O)C(=O)N3CC4=C(C3=C2)N=C5C=CC(=CC5=C4)O)O

InChi

InChI=1S/C20H16N2O5/c1-2-20(26)14-7-16-17-11(5-10-6-12(23)3-4-15(10)21-17)8-22(16)18(24)13(14)9-27-19(20)25/h3-7,23,26H,2,8-9H2,1H3

InChiKey

HAWSQZCWOQZXHI-UHFFFAOYSA-N

IUPAC Name

19-ethyl-7,19-dihydroxy-17-oxa-3,13-diazapentacyclo[11.8.0.02,11.04,9.015,20]henicosa-1(21),2(11),3,5,7,9,15(20)-heptaene-14,18-dione

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보완 제품

Assay Kits

AB65311

Cytochrome c Release Assay Kit

Functional Studies - Cytochrome c Release Assay Kit (AB65311)

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Biochemicals

AB120761

(R)-(-)-Phenylephrine hydrochloride, alpha1 Adrenoceptor agonist

Chemical Structure - (R)-(-)-Phenylephrine hydrochloride, alpha1 Adrenoceptor agonist (AB120761)

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Biochemicals

AB144183

Antibiotic PF 1052, Neutrophil migration inhibitor

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Assay Kits

AB156065

SIRT1 Activity Assay Kit (Fluorometric)

Functional Studies - SIRT1 Activity Assay Kit (Fluorometric) (AB156065)

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  • 2
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특성 및 보관 정보

배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
Store under desiccating conditions|The product can be stored for up to 12 months
취급 방법

Need more advice on solubility, usage and handling? Please visit our our product support page for more details.

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Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

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Toxic, refer to SDS for further information.

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제품 프로토콜

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Product promise

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