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AB120891

BIO, GSK3 inhibitor; cell-permeable

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MW 356.18 g/mol, Purity >98%. Highly potent, selective, reversible and ATP-competitive inhibitor of GSK3α/β (IC50 values are 5 (GSK3α/β), 83 (CDK5/p25), 300 (CDK2/cyclin A) and 320 nM (CDK1/cyclin B)). Activates the Wnt signaling pathway in vitro maintaining self-renewal and pluripotency in ESC. Cell-permeable.
2 이미지
Immunocytochemistry/ Immunofluorescence - BIO, GSK3 inhibitor; cell-permeable (AB120891)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - BIO, GSK3 inhibitor; cell-permeable (AB120891)

ab32572 staining ßcatenin in SW480 cells treated with BIO (ab120891), by ICC/IF. Increase of ßcatenin expression correlates with increased concentration of BIO, as described in literature.
The cells were incubated at 37°C for 48h in media containing different concentrations of ab120891 (BIO) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32572 (1/200) dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Chemical Structure - BIO, GSK3 inhibitor; cell-permeable (AB120891)
  • Chemical Structure

Lab

Chemical Structure - BIO, GSK3 inhibitor; cell-permeable (AB120891)

2D chemical structure image of ab120891, BIO, GSK3 inhibitor; cell-permeable

주요 정보

CAS 번호

667463-62-9

Purity

>98%

제형

Solid

form

Molecular weight

356.18 g/mol

Nature

Synthetic

Solubility

Soluble in DMSO to 25 mM

생물학적 정보

Highly potent, selective, reversible and ATP-competitive inhibitor of GSK3α/β (IC50 values are 5 (GSK3α/β), 83 (CDK5/p25), 300 (CDK2/cyclin A) and 320 nM (CDK1/cyclin B)). Activates the Wnt signaling pathway in vitro maintaining self-renewal and pluripotency in ESC. Cell-permeable.

Isomeric SMILES

O/N=C1/C(=C2C(=O)NC3CC(BR)CCC/23)NC2CCCCC12

IUPAC Name

(2'Z,3'E)-6-Bromoindirubin-3'-oxime

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보완 제품

Assay Kits

AB138893

Cell Tracking Dye Kit - Red - Cytopainter

Fluorescence Microscopy - Cell Tracking Dye Kit - Red - Cytopainter (AB138893)

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Assay Kits

AB138891

Cell Tracking Dye Kit - Green - Cytopainter

Fluorescence Microscopy - Cell Tracking Dye Kit - Green - Cytopainter (AB138891)

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Primary Antibodies

AB247142

Anti-WBSCR16 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WBSCR16 antibody (AB247142)

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Primary Antibodies

AB77232

Anti-Myoglobin antibody [EP3081Y]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myoglobin antibody [EP3081Y] (AB77232)

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특성 및 보관 정보

배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
보관 정보
It is important to note that this product is reported to be light sensitive|Store in the dark|Store under desiccating conditions
취급 방법

Need more advice on solubility, usage and handling? Please visit our our product support page for more details.

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Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

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Refer to SDS for further information.

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제품 프로토콜

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular neurobiology 61:5129-5141 PubMed38167971

2024

Palmitic Acid Induces Posttranslational Modifications of Tau Protein in Alzheimer's Disease-Related Epitopes and Increases Intraneuronal Tau Levels.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Melissa García-Cruz,Clorinda Arias
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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