DAF-FM DA, Nitric oxide quantification reagent

주요 정보

CAS 번호

254109-22-3

Purity

>98%

Molecular weight

496.42 g/mol

Molecular formula

C₂₅H₁₈F₂N₂O₇

Nature

Synthetic

Solubility

Soluble in DMSO

생물학적 정보

Cell permeable reagent for quantification of low concentrations of nitric oxide in solution. Essentially nonfluorescent until it is hydrolyzed to DAF-FM (ab145297) by intracellular esterases and reacts with NO to form a fluorescent benzotriazole.

InChi

InChI=1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3

InChiKey

BEVHTVRRVVEMEF-UHFFFAOYSA-N

IUPAC Name

3-Amino-4-(N-methylamino)-2',7'-difluorofluorescein diacetate

Excitation/Emission

Ex: 500nm, Em: 515nm