AB241082

Fluo-4 AM, Fluorescent labeling reagent

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(9 제품이 사용된 논문 )

Fluo-4 AM (ab241082)(CAS:273221-67-3) is a cell permeable green fluorescent calcium-binding dye binds to intracellular calcium (Kd = 345 nM ). Fluorescence intensity increases upon Ca²⁺ binding in live cells. Fluo-4 is the structural analog of Fluo-3.

- Excitation/Emission: Ex: 495 nm, Em: 528 nm
- Suitable for Flow cytometry and fluorescence microscopy
- BAPTA-Based Chelator: Fast calcium binding kinetics for real-time transient detection
- Available in different sizes to fit your experimental needs

1 이미지

Chemical Structure - Fluo-4 AM, Fluorescent labeling reagent (AB241082)

Chemical Structure

Supplier Data

Chemical Structure - Fluo-4 AM, Fluorescent labeling reagent (AB241082)

Chemical structure for Fluo-4 AM *Ultrapure Grade* CAS 273221-67-3

주요 정보

CAS 번호

273221-67-3

Purity

>98%

제형

Solid

form

Molecular weight

1096.9 Da

Molecular formula

C51H50F2N2O23

PubChem

4060965

Nature

Synthetic

Solubility

Soluble in DMSO to 1 mM.

생화학적 명칭

Fluo-4 am

생물학적 정보

A fluorescent labeling reagent for the detection of intracellular calcium. Binds to intracellular calcium (K_d = 345 nM). Fluo-4 is an analog of Fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.

Fluo-4 AM has spectral wavelength of maximum excitation @ ~490 nm and maximum emission @ ~520 nm.

Canonical SMILES

CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=CC(=C2)C3=C4C=C(C(=O)C=C4OC5=CC(=C(C=C53)F)OCOC(=O)C)F)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C

InChi

InChI=1S/C51H50F2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73-23-68-29(2)56/h7-10,13-18H,11-12,19-27H2,1-6H3

InChiKey

QOMNQGZXFYNBNG-UHFFFAOYSA-N

IUPAC Name

acetyloxymethyl 2-[2-[2-[5-[3-(acetyloxymethoxy)-2,7-difluoro-6-oxoxanthen-9-yl]-2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]phenoxy]ethoxy]-N-[2-(acetyloxymethoxy)-2-oxoethyl]-4-methylanilino]acetate

Excitation/Emission

Ex: 494nm, Em: 516nm

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Fluorescent Cell Imaging": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Chemical Structure": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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제품 세부 정보

Check out our range of Fluo AM dyes here.

Related Products:
Calcium indicators and ionophores

Protocol:
1. On the day of the experiment, either dissolve Fluo-4 AM in DMSO or thaw an aliquot of the indicator stock solution to room temperature.
2. Prepare a 2 to 20 µM Fluo-4 AM working solution in a buffer of your choice (e.g., Hanks and Hepes buffer) with 0.04% Pluronic^® F-127. For most cell lines, Fluo-4 AM at a final concentration of 4-5 μM is recommended. The exact concentration of indicators required for cell loading must be determined empirically.

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특성 및 보관 정보

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

보관 정보

Store in the dark|Store under desiccating conditions

취급 방법

Need more advice on solubility, usage and handling? Please visit our our product support page for more details.

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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제품이 사용된 논문 (9)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 16:3012 PubMed40148355

2025

Medium from human iPSC-derived primitive macrophages promotes adult cardiomyocyte proliferation and cardiac regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Xiao,Hao Zhang,Xu Liu,Pengfei Xu,Heng Du,Jiawan Wang,Jianghua Shen,Yujing Li,Yuhan Wang,Chuting He,Haiping Feng,Jingfang Liu,Yanan Zhou,Siqi Liu,Zeyu Gao,Jingyi Zang,Jinmiao Bi,Tie-Shan Tang,Qi Gu,Tuo Wei,Jun Wang,Moshi Song

NPJ Parkinson's disease 10:138 PubMed39069518

2024

Human tripartite cortical network model for temporal assessment of alpha-synuclein aggregation and propagation in Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Fikret Emre Kapucu,Iisa Tujula,Oskari Kulta,Lassi Sukki,Tomi Ryynänen,Hjalte Gram,Valtteri Vuolanto,Andrey Vinogradov,Joose Kreutzer,Poul Henning Jensen,Pasi Kallio,Susanna Narkilahti

Cellular and molecular life sciences : CMLS 81:217 PubMed38748186

2024

Membrane-bound chemoreception of bitter bile acids and peptides is mediated by the same subset of bitter taste receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Schaefer,Florian Ziegler,Tatjana Lang,Alexandra Steuer,Antonella Di Pizio,Maik Behrens

Biochimica et biophysica acta. Molecular basis of disease 1869:166793 PubMed37336366

2023

Apolipoprotein E ε4 triggers neurotoxicity via cholesterol accumulation, acetylcholine dyshomeostasis, and PKCε mislocalization in cholinergic neuronal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca Piccarducci,Chiara Giacomelli,Maria Sofia Bertilacchi,Andrea Benito-Martinez,Nicoletta Di Giorgi,Simona Daniele,Giovanni Signore,Silvia Rocchiccioli,Marçal Vilar,Laura Marchetti,Claudia Martini

Cell communication and signaling : CCS 21:132 PubMed37316873

2023

Simultaneous induction of vasculature and neuronal network formation on a chip reveals a dynamic interrelationship between cell types.

Applications

Unspecified application

Species

Unspecified reactive species

Lotta Isosaari,Hanna Vuorenpää,Alma Yrjänäinen,Fikret Emre Kapucu,Minna Kelloniemi,Toni-Karri Pakarinen,Susanna Miettinen,Susanna Narkilahti

Scientific reports 11:23232 PubMed34853378

2021

Therapeutic potential of TRPM8 antagonists in prostate cancer.

Applications

Fluorescent Cell Imaging

Species

Human

Marzia Di Donato,Carmine Ostacolo,Pia Giovannelli,Veronica Di Sarno,Isabel M Gomez Monterrey,Pietro Campiglia,Antimo Migliaccio,Alessia Bertamino,Gabriella Castoria

ACS omega 6:29664-29674 PubMed34778638

2021

New Antagonists of the Membrane Androgen Receptor OXER1 from the ZINC Natural Product Database.

Applications

Unspecified application

Species

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Athanasios A Panagiotopoulos,Konstantina Kalyvianaki,George Notas,Stergios A Pirintsos,Elias Castanas,Marilena Kampa

Molecular metabolism 54:101329 PubMed34454092

2021

An inhibitor-mediated beta-cell dedifferentiation model reveals distinct roles for FoxO1 in glucagon repression and insulin maturation.

Applications

Unspecified application

Species

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Tamara Casteels,Yufeng Zhang,Thomas Frogne,Caterina Sturtzel,Charles-Hugues Lardeau,Ilke Sen,Xiaocheng Liu,Shangyu Hong,Florian M Pauler,Thomas Penz,Marlene Brandstetter,Charlotte Barbieux,Ekaterine Berishvili,Thomas Heuser,Christoph Bock,Christian G Riedel,Dirk Meyer,Martin Distel,Jacob Hecksher-Sørensen,Jin Li,Stefan Kubicek

iScience 24:102535 PubMed34124613

2021

pneumolysin and neuraminidase A convert high-density lipoproteins into pro-atherogenic particles.

Applications

Unspecified application

Species

Unspecified reactive species

Shahan Syed,Eija Nissilä,Hanna Ruhanen,Satoshi Fudo,Meztlli O Gaytán,Sanna P Sihvo,Martina B Lorey,Jari Metso,Katariina Öörni,Samantha J King,Oommen P Oommen,Matti Jauhiainen,Seppo Meri,Reijo Käkelä,Karita Haapasalo

제품이 사용된 논문 모두 보기

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Product promise

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Fluo-4 AM, Fluorescent labeling reagent

Chemical Structure - Fluo-4 AM, Fluorescent labeling reagent (AB241082)

주요 정보

CAS 번호

Purity

제형

Molecular weight

Molecular formula

PubChem

Nature

Solubility

생화학적 명칭

생물학적 정보

Canonical SMILES

InChi

InChiKey

IUPAC Name

Excitation/Emission

보완 제품

Biochemicals

Biochemicals

Secondary Antibodies

Biochemicals

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

취급 방법

제품 프로토콜

제품이 사용된 논문 (9)

Product promise