AB120873

Fura-2 AM, Ca2+ selective fluorescent indicator

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(31 제품이 사용된 논문 )

Fura-2 AM (ab120873) is a high affinity Ca2+ selective, cell-permeable fluorescent indicator (Kd = 145 nM). Rapidly metabolized by cytoplasmic esterases leading to the active dye Fura 2. MW 1001.8.

- Excitation spectrum dependent on [Ca2+]i. Ex: 340/380 nm. Em: 505 nm
- Proven performance: cited in over 30 publications

3 이미지

Chemical Structure - Fura-2 AM, Ca2+ selective fluorescent indicator (AB120873)

Chemical Structure

Lab

Chemical Structure - Fura-2 AM, Ca2+ selective fluorescent indicator (AB120873)

2D chemical structure image of ab120873, Fura-2 AM, Ca2+ selective fluorescent indicator

Fluorescent Cell Imaging - Fura-2 AM, Ca2+ selective fluorescent indicator (AB120873)

Fluorescent Cell Imaging

Lab

Fluorescent Cell Imaging - Fura-2 AM, Ca2+ selective fluorescent indicator (AB120873)

Direct comparison of Abcam and competitor's Fura-2 AM. RAW 264.7 cells were loaded with 2 μM of Fura-2 AM from Abcam (ab120873) and competitor's in a DMSO/ HBSS solution for 30 minutes at room temperature and in the dark. After performing two washes with pre-warmed HBSS the cells were further incubated for 30 minutes at 37°C in the dark. Finally, washes with pre-warmed HBSS were performed and the live cells were imaged on a confocal microscope (using the same exposure settings) : excitation laser = 405nm, emission gate center = 519nm.

Fluorescent Cell Imaging

Lab

Fluorescent Cell Imaging - Fura-2 AM, Ca2+ selective fluorescent indicator (AB120873)

Direct comparison of Abcam and competitor's Fura-2 AM. RAW 264.7 cells were loaded with different concentrations of Fura-2 AM from Abcam (ab120873) and competitor's in a DMSO/ HBSS solution for 30 minutes at room temperature and in the dark. After performing two washes with pre-warmed HBSS the cells were further incubated for 30 minutes at 37°C in the dark. Finally, washes with pre-warmed HBSS were performed and the live cells were imaged on a confocal microscope (using the same exposure settings) : excitation laser = 405nm, emission gate center = 519nm.

주요 정보

CAS 번호

108964-32-5

Purity

>95%

제형

Solid

form

Molecular weight

1001.8 Da

Molecular formula

C44H47N3O24

PubChem

3364574

Nature

Synthetic

Solubility

Soluble in DMSO to 10 mM

생화학적 명칭

Fura 2-AM

생물학적 정보

High affinity Ca2+ selective fluorescent indicator (Kd = 145 nM). Rapidly metabolized by cytoplasmic esterases leading to the active dye Fura 2. Cell-permeable. Excitation spectrum dependent on [Ca2+]i. Ex340/380/Em505.

Canonical SMILES

CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=C3C(=C2)C=C(O3)C4=NC=C(O4)C(=O)OCOC(=O)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C

InChi

InChI=1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,16-24H2,1-6H3

InChiKey

VPSRLGDRGCKUTK-UHFFFAOYSA-N

IUPAC Name

acetyloxymethyl 2-[6-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-5-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-1-benzofuran-2-yl]-1,3-oxazole-5-carboxylate

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제품 세부 정보

1 unit = 50 µg

1 mg - 1 x 1 mg vial
20 units - 20 x 50 µg vials
10 units - 10 x 50 µg vials
50 µg - 1 x 50 µg vial

Related protocols:
Fura-2 AM imaging protocol

Related Products:
Calcium indicators and ionophores

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특성 및 보관 정보

배송 시 보관 조건

Ambient - Can Ship with Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

보관 정보

Store under desiccating conditions|The product can be stored for up to 12 months

취급 방법

Need more advice on solubility, usage and handling? Please visit our our product support page for more details.

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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제품이 사용된 논문 (31)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of molecular and cellular cardiology plus 12:100301 PubMed40485773

2025

PP2A-B56α is a key determinant of cardiac protein phosphorylation and functional responses to β-adrenergic signalling.

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Alican Güran,Yanlong Ji,Pan Fang,Kuan-Ting Pan,Henning Urlaub,Metin Avkiran,Christof Lenz,Kate L Weeks

International journal of molecular sciences 26: PubMed40244142

2025

MnO Nanocrystal-Induced Eryptosis Features Ca Overload, ROS and RNS Accumulation, Calpain Activation, Recruitment of Caspases, and Changes in the Lipid Order of Cell Membranes.

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Yuriy Kot,Volodymyr Prokopiuk,Vladimir Klochkov,Liliya Tryfonyuk,Pavel Maksimchuk,Andrey Aslanov,Kateryna Kot,Oleg Avrunin,Lesya Demchenko,Saulesh Kurmangaliyeva,Anatolii Onishchenko,Svetlana Yefimova,Ondrej Havranek,Anton Tkachenko

International journal of molecular sciences 26: PubMed40003936

2025

Niemann-Pick C-like Endolysosomal Dysfunction in DHDDS Patient Cells, a Congenital Disorder of Glycosylation, Can Be Treated with Miglustat.

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Hannah L Best,Sophie R Cook,Helen Waller-Evans,Emyr Lloyd-Evans

International journal of molecular sciences 25: PubMed39519237

2024

GdVO:Eu and LaVO:Eu Nanoparticles Exacerbate Oxidative Stress in L929 Cells: Potential Implications for Cancer Therapy.

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Yuriy Kot,Vladimir Klochkov,Volodymyr Prokopiuk,Olha Sedyh,Liliya Tryfonyuk,Ganna Grygorova,Nina Karpenko,Oleksandr Tomchuk,Kateryna Kot,Anatolii Onishchenko,Svetlana Yefimova,Anton Tkachenko

Cell communication and signaling : CCS 22:533 PubMed39506805

2024

Breast cancer cells utilize T3 to trigger proliferation through cellular Ca modulation.

Applications

Unspecified application

Species

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Ines Tawfik,Katharina Schlick,Julian Ostaku,Doruntina Bresilla,Sonja Gabrijelčič,Benjamin Gottschalk,Alwin Sokolowski,Ernst Malle,Katarina Kalinova,Martin Hirtl,Corina T Madreiter-Sokolowski

International journal of molecular sciences 25: PubMed39273556

2024

Left Ventricular Systolic Dysfunction in NBCe1-B/C-Knockout Mice.

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Unspecified application

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Clayton T Brady,Aniko Marshall,Lisa A Eagler,Thomas M Pon,Michael E Duffey,Brian R Weil,Jennifer K Lang,Mark D Parker

Scientific reports 14:10877 PubMed38740862

2024

Astroglial connexin 43 is a novel therapeutic target for chronic multiple sclerosis model.

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Ezgi Ozdemir Takase,Ryo Yamasaki,Satoshi Nagata,Mitsuru Watanabe,Katsuhisa Masaki,Hiroo Yamaguchi,Jun-Ichi Kira,Hideyuki Takeuchi,Noriko Isobe

Translational neuroscience 15:20220341 PubMed38736723

2024

Suanzaoren decoction exerts its antidepressant effect via the CaMK signaling pathway.

Applications

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Species

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Xiaofang Zhang,Jiyuan Guo,Ce Zhang,Wenhua Wang,Shuailin Du,Xusheng Tian

Nature neuroscience 27:822-835 PubMed38589584

2024

Neuronal activity rapidly reprograms dendritic translation via eIF4G2:uORF binding.

Applications

Unspecified application

Species

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Ezgi Hacisuleyman,Caryn R Hale,Natalie Noble,Ji-Dung Luo,John J Fak,Misa Saito,Jin Chen,Jonathan S Weissman,Robert B Darnell

Experimental physiology 109:711-728 PubMed38500268

2024

Time course changes in in vivo muscle mechanical function and Ca regulation of force following experimentally induced gradual ovarian failure in mice.

Applications

Unspecified application

Species

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Avery Hinks,Benjamin E Dalton,Parastoo Mashouri,Luke D Flewwelling,William Glen Pyle,Arthur J Cheng,Geoffrey A Power

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Fura-2 AM, Ca2+ selective fluorescent indicator

Chemical Structure - Fura-2 AM, Ca2+ selective fluorescent indicator (AB120873)

Fluorescent Cell Imaging - Fura-2 AM, Ca2+ selective fluorescent indicator (AB120873)

Fluorescent Cell Imaging - Fura-2 AM, Ca2+ selective fluorescent indicator (AB120873)

주요 정보

CAS 번호

Purity

제형

Molecular weight

Molecular formula

PubChem

Nature

Solubility

생화학적 명칭

생물학적 정보

Canonical SMILES

InChi

InChiKey

IUPAC Name

보완 제품

Biochemicals

Biochemicals

Reagents, Controls & Accessories

Assay Kits

제품 세부 정보

특성 및 보관 정보

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

취급 방법

제품 프로토콜

제품이 사용된 논문 (31)

Product promise