Galanin (1-29) (rat, mouse), Galanin receptor agonist
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- FuncS
Unknown
Functional Studies - Galanin (1-29) (rat, mouse), Galanin receptor agonist (AB141153)
ab81283 staining AKT1 (phospho S473) in PC12 cells treated with galanin (1-29) (rat, mouse) (ab141153), by ICC/IF. Increase of AKT1 (phospho S473) expression correlates with increased concentration of galanin (1-29) (rat, mouse), as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab141153 (galanin (1-29) (rat, mouse)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab81283 (1/100) dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight® 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
- Chemical Structure
Lab
Chemical Structure - Galanin (1-29) (rat, mouse), Galanin receptor agonist (AB141153)
2D chemical structure image of ab141153, Galanin (1-29) (rat, mouse), Galanin receptor agonist
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