AB156034

10X RIPA Buffer

Name: 10X RIPA Buffer
Brand: Abcam Limited
SKU: AB156034
Rating: 5 (2 reviews)

(2 리뷰들)

(246 제품이 사용된 논문 )

10X RIPA lysis buffer (pH 7.5) (ab156034) is used for cell lysis and protein solubilization for both adherent and suspension-cultured mammalian cells and tissues. Phosphatase inhibitors included in buffer formulation.

- Suitable buffer for your western blot SDS-PAGE, ELISA, IP and BCA assays
- Useful for lysis of whole-cell extracts and membrane-bound proteins
- Proven performance: cited in over 245 publications.
- Larger volume format available upon request

1 이미지

SDS-PAGE

Unknown

SDS-PAGE - 10X RIPA Buffer (AB156034)

HeLa cell extraction using ab156034.
2.5 million HeLa cells were lysed on ice for 15 minutes with 0.5 mL of 1X ab156034. Next the sample was centrifuged at 14,000 rpm at 4ºC for 15 minutes : the supernatant ( = cleared lysate) was removed and the pellet ( = insoluble material) was resuspended in 0.5 mL lysis buffer and solubilized by sonication. Equivalent loads of the cleared lysate and solubilized pellet were analyzed by SDS-PAGE and Coomassie stain.
BCA protein concentration determination of the soluble and insoluble material indicates that a total of 1.1mg of protein was recovered and 82% was in the soluble cleared cell lysate.

Lane 1 : MW marker
Lane 2 : Cleared lysate
Lane 3 : Non-soluble

주요 정보

Applications

WB, IP, ELISA, SDS-PAGE

applications

제형

Liquid

form

보관 버퍼

pH: 7.5 Constituents: 10% Nonylphenol, ethoxylated, 8.8% Sodium chloride, 6.1% Tris, 5% Sodium deoxycholate, 1.12% Sodium pyrophosphate decahydrate, 1% Sodium lauryl sulfate, 0.38% EGTA, 0.29% EDTA, 0.22% disodium;1,3-dihydroxypropan-2-yl phosphate;hydrate, 0.18% Sodium orthovanadate

storage-buffer

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IP": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Suggested working concentration: 1X" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Suggested working concentration: 1X" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Suggested working concentration: 1X" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Suggested working concentration: 1X" } } }

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제품 세부 정보

Abcam's 10X RIPA lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells. This reagent effectively extracts cytoplasmic, nuclear and membrane proteins. It is compatible with many downstream applications, including SDS-PAGE, Western blot, immunoprecipitation, ELISA and BCA assays.

Preparation: Dilute to 1X in deionized water

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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특성 및 보관 정보

배송 시 보관 조건

Ambient - Cannot Ship with Ice

적절한 단기 보관 조건

Ambient

적절한 장기 보관 조건

Ambient

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

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제품이 사용된 논문 (246)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cytotechnology 77:137 PubMed40621114

2025

Mechanistic insights into Aloin-mediated regulation of STAT3/SLC7A11 signaling in head and neck squamous cell carcinoma.

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NPJ precision oncology 9:224 PubMed40615519

2025

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Applications

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Species

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Sreya Das,Daniel S Lenchner,Ellen Jaeger,Lisa Hunihan,Dana F DeSantis,Stamatina Fragkogianni,Karyn Ronski,Frederick H Wilson

Scientific reports 15:20470 PubMed40595762

2025

Polysaccharide from Prinsepia utilis Royle maintains the skin barrier by mediating differentiation, lipid metabolism and tight junction of keratinocyte.

Applications

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Ying Tu,Na Li,Hai-Yang Liu,Dong-Jie Sun,Li He,Hua Gu

Frontiers in pharmacology 16:1591394 PubMed40538548

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Aqueous extracts of L. alleviate atopic dermatitis by restoring skin barrier function.

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Haobin Wei,Zhang Chen,Wenjie Lai,Wenxian Wang,Xiqing Bian,Limin Zhang,Xinzhi Li

Methods in molecular biology (Clifton, N.J.) 2921:255-264 PubMed40515994

2025

Isolation of Exosome from Senescent Preadipocytes for Mass Spectrometric Analysis.

Applications

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Species

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Yao Wang

Genome research 35:1456-1471 PubMed40360186

2025

Charting the regulatory landscape of TP53 on transposable elements in cancer.

Applications

Unspecified application

Species

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Xuan Qu,Yonghao Liang,Colin McCornack,Xiaoyun Xing,Heather Schmidt,Chad Tomlinson,Catrina Fronick,Edward A Belter,Juan F Macias-Velasco,Ting Wang

Nature communications 16:4393 PubMed40355429

2025

SARS-CoV-2 ORF3a drives dynamic dense body formation for optimal viral infectivity.

Applications

Unspecified application

Species

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Stella Hartmann,Lisa Radochonski,Chengjin Ye,Luis Martinez-Sobrido,Jueqi Chen

International journal of molecular sciences 26: PubMed40332529

2025

VEGF in Tears as a Biomarker for Exudative Age-Related Macular Degeneration: Molecular Dynamics in a Mouse Model and Human Samples.

Applications

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Species

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Seyed Mohamad Mehdi Moshtaghion,Filippo Locri,Alvaro Plaza Reyes,Flavia Plastino,Anders Kvanta,Maria Jose Morillo-Sanchez,Enrique Rodríguez-de-la-Rúa,Estanislao Gutierrez-Sanchez,Adoración Montero-Sánchez,Helena Lucena-Padros,Helder André,Francisco J Díaz-Corrales

Cell & bioscience 15:55 PubMed40281637

2025

Zmynd11 is essential for neurogenesis by coordinating H3K36me3 modification of Epha2 and PI3K signaling pathway.

Applications

Unspecified application

Species

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Xu Yang,Lan Li,Wenzheng Qu,Xuejun Cheng,Jinyu Zhang,Yan Sun,Suxiao Liu,Guoping Peng,Rui Zheng,Xuekun Li

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2409040 PubMed40245004

2025

α-Synuclein Pathology Spreads in a Midbrain-Hindbrain Assembloid Model.

Applications

Unspecified application

Species

Unspecified reactive species

Gemma Gomez-Giro,Daniela Frangenberg,Daniela Vega,Alise Zagare,Kyriaki Barmpa,Paul M A Antony,Graham Robertson,Rahman Sabahi-Kaviani,Kristian Haendler,Nathalie Kruse,Florentia Papastefanaki,Rebecca Matsas,Malte Spielmann,Regina Luttge,Jens C Schwamborn

제품이 사용된 논문 모두 보기

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

10X RIPA Buffer

SDS-PAGE - 10X RIPA Buffer (AB156034)

주요 정보

Applications

제형

보관 버퍼

보완 제품

Reagents, Controls & Accessories

Secondary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

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타겟 정보

제품이 사용된 논문 (246)

Product promise