ATRX KO cell line available to order. KO validated. Free of charge wild type control available. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
대체 명칭 보기
ATRX, X-linked helicase II, ATP-dependent helicase ATRX, Transcriptional regulator ATRX, RAD54L, XH2, X-linked nuclear protein, Znf-HX, XNP
- WB
Lab
Western blot - Human ATRX knockout HCT116 cell line (AB287231)
Western blot : Anti-ATRX antibody staining at 0.4 ug/ml, shown in black; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to ATRX. A band was observed at 150/180/350 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in ATRX knockout cell line ab287231. To generate this image, wild-type and ATRX knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged with 12 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes:
Western blot - Anti-ATRX antibody (<a href='/ko/products/primary-antibodies/atrx-antibody-ab204169'>ab204169</a>) at 0.4 µg/mL
Lane 1:
Wild-type HCT 116 cell lysate at 20 µg
Lane 2:
Western blot - Human ATRX knockout HCT116 cell line (ab287231)
Lane 2:
HCT 116 cell lysate at 20 µg
Lane 3:
U-2 OS cell lysate at 20 µg
Secondary
All lanes:
HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Predicted band size: 282 kDa
false
- NGS
Lab
Next Generation Sequencing - Human ATRX knockout HCT116 cell line (AB287231)
2 bp deletion after the 341st AA of the WT protein
보완 제품
Primary Antibodies
AB316744
Anti-CSB antibody [EPR27019-44]
20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size
![Immunoprecipitation - Anti-CSB antibody [EPR27019-44] (AB316744)](https://content.abcam.com/products/images/csb-antibody-epr27019-44-ab316744--immunoprecipitation-img428585.jpg)
- 0
- 0
Primary Antibodies
AB76420
Anti-RPA32/RPA2 antibody [EPR2877Y]
20ul selling size|RabMAb|Recombinant
![Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)](https://content.abcam.com/products/images/rpa32-rpa2-antibody-epr2877y-ab76420--immunocytochemistry-immunofluorescence-img33036.jpg)
- 5
- 1
제품 세부 정보
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control: Human wild-type HCT116 cell line (ab288559). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ATRX functions by forming part of the ATRX-DAXX complex. This complex plays a vital role in the deposition of the histone variant H3.3 into heterochromatin regions affecting the structure and function of these genomic segments. ATRX through the ATRX-DAXX complex also maintains telomere integrity by preventing uncontrolled telomere elongation. As such ATRX is important in cellular processes like DNA replication repair and mitotic progression emphasizing its role in maintaining genetic fidelity across cell divisions.
Pathways
ATRX is an important component in chromatin remodeling and telomere maintenance pathways. It interacts closely with the DAXX protein within these pathways affecting the histone exchange mechanisms. ATRX also engages with other proteins such as p53 integrating its function into the DNA damage repair pathway. Through these pathways ATRX ensures proper chromatin dynamics and participates in mechanisms guarding genomic stability.
품질 관리
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
초기 취급 지침
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability. <p>1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.<br>2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.<br>3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.<br>4. Incubate the culture at 37°C incubator with 5% CO<sub>2</sub>. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.<br>5. Once confluent passage into an appropriate flask at a density of 2x10<sup>4</sup> cells/cm<sup>2</sup>. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.</p>
Subculture 가이드라인
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
배양 배지
McCoY5a + 10% FBS
동결보존 배지
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com