Human CD19 knockout Raji cell line (ab274913) available to order. Recommended control: Human wild-type Raji cell line (ab271145).
- CD19 KO validation: Next Generation Sequencing (NGS)
- Concentration: 1 million cells/vial
대체 명칭 보기
CD19, B-lymphocyte antigen CD19, Differentiation antigen CD19, T-cell surface antigen Leu-12, B-lymphocyte surface antigen B4
- NGS
Supplier Data
Next Generation Sequencing - Human CD19 knockout Raji cell line (AB274913)
Knockout achieved by CRISPR/Cas9; X = 2 bp deletion; Frameshift : 100%
- WB
Lab
Western blot - Human CD19 knockout Raji cell line (AB274913)
Western blot : Anti-CD19 antibody [RM1166] ab317335 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 80-90 kDa in Wild-type Raji cell lysates with no signal observed at this size in CD19 knockout Raji cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.
All lanes:
Western blot - Anti-CD19 antibody [RM2072] (<a href='/ko/products/primary-antibodies/cd19-antibody-rm2072-ab322345'>ab322345</a>) at 1/1000 dilution
Lane 1:
Wild-type Raji at 20 µg
Lane 2:
Western blot - Human CD19 knockout Raji cell line (ab274913) at 20 µg
Lane 3:
Ramos at 20 µg
Lane 4:
MCF7 at 20 µg
Lane 5:
Daudi at 20 µg
Lane 6:
Jurkat at 20 µg
Secondary
All lanes:
Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution
Predicted band size: 80-90 kDa
Observed band size: 80-90 kDa
false
- WB
Lab
Western blot - Human CD19 knockout Raji cell line (AB274913)
Western blot : Anti-CD19 antibody [EPR5906] ab134114 staining at 1/10000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 80-90 kDa in Wild-type Raji cell lysates with no signal observed at this size in CD19 knockout Raji cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.
All lanes:
Western blot - Anti-CD19 antibody [EPR5906] (<a href='/ko/products/primary-antibodies/cd19-antibody-epr5906-ab134114'>ab134114</a>) at 1/10000 dilution
Lane 1:
Wild-type Raji at 20 µg
Lane 2:
Western blot - Human CD19 knockout Raji cell line (ab274913) at 20 µg
Lane 3:
Ramos at 20 µg
Lane 4:
MCF7 at 20 µg
Lane 5:
Daudi at 20 µg
Lane 6:
Jurkat at 20 µg
Secondary
All lanes:
Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution
Predicted band size: 80-90 kDa
Observed band size: 80-90 kDa
false
- WB
Lab
Western blot - Human CD19 knockout Raji cell line (AB274913)
Western blot : Anti-CD19 antibody [SP291] - C-terminal ab227688 staining at 1/400 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 80-90 kDa in Wild-type Raji cell lysates with no signal observed at this size in CD19 knockout Raji cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.
All lanes:
Western blot - Anti-CD19 antibody [SP291] - C-terminal (<a href='/ko/products/primary-antibodies/cd19-antibody-sp291-c-terminal-ab227688'>ab227688</a>) at 1/400 dilution
Lane 1:
Wild-type Raji at 20 µg
Lane 2:
Western blot - Human CD19 knockout Raji cell line (ab274913) at 20 µg
Lane 3:
Ramos at 20 µg
Lane 4:
MCF7 at 20 µg
Lane 5:
Daudi at 20 µg
Lane 6:
Jurkat at 20 µg
Secondary
All lanes:
Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution
Predicted band size: 80-90 kDa
Observed band size: 80-90 kDa
false
- WB
Lab
Western blot - Human CD19 knockout Raji cell line (AB274913)
Western blot : Anti-CD19 antibody [RM1166] ab317335 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 80-90 kDa in Wild-type Raji cell lysates with no signal observed at this size in CD19 knockout Raji cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.
All lanes:
Western blot - Anti-CD19 antibody [RM1166] (<a href='/ko/products/primary-antibodies/cd19-antibody-rm1166-ab317335'>ab317335</a>) at 1/1000 dilution
Lane 1:
Wild-type Raji at 20 µg
Lane 2:
Western blot - Human CD19 knockout Raji cell line (ab274913) at 20 µg
Lane 3:
Ramos at 20 µg
Lane 4:
MCF7 at 20 µg
Lane 5:
Daudi at 20 µg
Lane 6:
Jurkat at 20 µg
Secondary
All lanes:
Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution
Predicted band size: 80-90 kDa
Observed band size: 80-90 kDa
false
- WB
Lab
Western blot - Human CD19 knockout Raji cell line (AB274913)
Western blot : Anti-CD19 antibody [4C7] ab288365 staining at 1/2000 dilution, shown in green; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 80-90 kDa in Wild-type Raji cell lysates with no signal observed at this size in CD19 knockout Raji cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.
All lanes:
Western blot - Anti-CD19 antibody [4C7] (<a href='/ko/products/primary-antibodies/cd19-antibody-4c7-ab288365'>ab288365</a>) at 1/2000 dilution
Lane 1:
Wild-type Raji at 20 µg
Lane 2:
Western blot - Human CD19 knockout Raji cell line (ab274913) at 20 µg
Lane 3:
Ramos at 20 µg
Lane 4:
MCF7 at 20 µg
Lane 5:
Daudi at 20 µg
Lane 6:
Jurkat at 20 µg
Secondary
All lanes:
Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution
Predicted band size: 80-90 kDa
Observed band size: 80-90 kDa
false
보완 제품
Reactivity 정보
제품 세부 정보
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
Knockout 검증
배송 시 보관 조건
적절한 단기 보관 기간
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD19 acts as an essential player in B cell signaling. CD19 integrates signals from the BCR with other coreceptors acting as a signaling hub. It participates in forming a signaling complex that includes proteins like CD21 and CD81 which further amplifies BCR-mediated signaling. This complex plays a critical role in B cell activation differentiation and survival ensuring that immune responses are efficiently mounted against pathogens.
Pathways
The CD19 protein fits into key immune response pathways such as the BCR signaling pathway and the PI3K-Akt signaling pathway. CD19 collaborates with other proteins like CD21 CD81 and PI3K within these pathways. Its interaction in the PI3K-Akt pathway for instance supports critical processes including B cell activation and homeostasis contributing to the adaptive immune response.
품질 관리
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Suspension
초기 취급 지침
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability. <p>1. Thaw the vial in 37°C water for bath approximately 1-2 minutes.<br>2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.<br>3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 4x10<sup>5</sup> - 5x10<sup>5</sup> cells/mL(for initial passages it is recomended to culture the cells in the higher range of recomended seeding density). Seeding density is given as a guide only and should be scaled to align with individual lab schedules.<br>4. Incubate the culture at 37°C incubator with 5% CO<sub>2</sub>. Cultures should be monitored daily.</p>
Subculture 가이드라인
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 4x105 cells/mL is recommended.
- A maximum of 3x106 viable cells/mL is obtainable.
배양 배지
RPMI + 10% FBS
동결보존 배지
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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