Human GATA1 knockout K562 cell line[C116]
- Advanced Validation
- 각 태그의 뜻
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GATA1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Sanger sequencing of this cell line reveals two indels with equal contribution: -34bp and -36bp from the beginning of exon 2, spanning across the intron/exon border (clone 67). Knockout. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
대체 명칭 보기
GF1, GATA-binding factor 1, GATA-1, ERYF1, GF-1, GATA1, NF-E1 DNA-binding protein, Erythroid transcription factor, Eryf1
![Western blot - Human GATA1 knockout K562 cell line[C116] (AB285360)](https://content.abcam.com/products/images/human-gata1-knockout-k562-cell-linec116-ab285360--western-blot-img435356.jpg/_jcr_content/renditions/webp-768.webp)
- WB
Lab
Western blot - Human GATA1 knockout K562 cell line[C116] (AB285360)
Western blot : Anti-GATA1 antibody [EPR17362] (ab181544) staining at 1/10000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab181544 was shown to bind specifically to GATA1. A band was observed at 45 kDa in wild-type K562 cell lysates with no signal observed at this size in GATA1 knockout cell line ab285360 (knockout K562 cell lysate). To generate this image, wild-type and GATA1 knockout K562 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes:
Western blot - Anti-GATA1 antibody [EPR17362] - ChIP Grade (<a href='/ko/products/primary-antibodies/gata1-antibody-epr17362-chip-grade-ab181544'>ab181544</a>) at 1/10000 dilution
Lane 1:
Wild-type K562 cell lysate at 20 µg
Lane 2:
GATA1 knockout K562 cell lysate at 20 µg
Secondary
All lanes:
Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Observed band size: 45 kDa
false
보완 제품
Primary Antibodies
AB181544
Anti-GATA1 antibody [EPR17362] - ChIP Grade
20ul selling size|KO Validated|RabMAb|Recombinant
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GATA1 antibody [EPR17362] - ChIP Grade (AB181544)](https://content.abcam.com/products/images/gata1-antibody-epr17362-chip-grade-ab181544--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130512.jpg)
- 0
- 0
Primary Antibodies
AB32147
RabMAb|Recombinant|KO Validated|20ul selling size
![Western blot - Anti-Cdk2 antibody [E304] (AB32147)](https://content.abcam.com/products/images/cdk2-antibody-e304-ab32147--western-blot-img161728.jpg)
- 5
- 8
Reactivity 정보
제품 세부 정보
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
Knockout 검증
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GATA1 regulates gene expression and is essential for erythroid and megakaryocyte differentiation. GATA1 belongs to a family of GATA transcription factors and can form regulatory complexes with other proteins such as FOG1 (Friend of GATA1) and TAL1. These complexes facilitate the binding of GATA1 to DNA enhancing its transcriptional activity on target gene sets necessary for blood cell maturation and function.
Pathways
GATA1 participates in the erythropoiesis and megakaryopoiesis pathways. It directly regulates several genes and coordinates signals with factors like GATA2 and RUNX1 which are other key proteins involved in these pathways. Through these interactions GATA1 ensures the correct development of red blood cells and platelets maintaining hematopoietic homeostasis in the organism.
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Female
초기 취급 지침
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability. <p>1. Thaw the vial in 37°C water for bath approximately 1-2 minutes. <br>2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution. <br>3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x10<sup>5</sup> cells/mL. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. <br>4. Incubate the culture at 37°C incubator with 5% CO<sub>2</sub>. Cultures should be monitored daily.</p>
Subculture 가이드라인
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x105 cells/mL is recommended.
- Subculture when cells reach 1x106 cells/mL.
배양 배지
IMDM + 10% FBS
동결보존 배지
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
제품이 사용된 논문 (1)
Recent publications for all applications. Explore the 전체 목록 and refine your search
Molecular therapy. Nucleic acids 7:288-298 PubMed28624204
2017
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
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