Human HPDL knockout HeLa cell line
- Advanced Validation
- 각 태그의 뜻
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- WB
Lab
Western blot - Human HPDL knockout HeLa cell line (AB265868)
Lanes 1-3 : Merged signal (red and green). Green - ab174841 observed at 39 kDa. Red - loading control ab7291 observed at 50 kDa.
ab174841 Anti-HPDL antibody [EPR11691] - C-terminal was shown to specifically react with HPDL in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265868 (knockout cell lysate ab257995) was used. Wild-type and HPDL knockout samples were subjected to SDS-PAGE. ab174841 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-HPDL antibody [EPR11691] - C-terminal (<a href='/ko/products/primary-antibodies/hpdl-antibody-epr11691-c-terminal-ab174841'>ab174841</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
HPDL knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human HPDL knockout HeLa cell line (ab265868)
Lane 3:
Caco-2 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human HPDL knockout HeLa cell line (AB265868)
Allele-1 : 2 bp deletion in exon 1.
- Sanger seq
Unknown
Sanger Sequencing - Human HPDL knockout HeLa cell line (AB265868)
Allele-2 : 1 bp insertion in exon 1.
보완 제품
Primary Antibodies
AB174841
Anti-HPDL antibody [EPR11691] - C-terminal
RabMAb|Recombinant|KO Validated
![Immunoprecipitation - Anti-HPDL antibody [EPR11691] - C-terminal (AB174841)](https://content.abcam.com/products/images/hpdl-antibody-epr11691-c-terminal-ab174841--immunoprecipitation-img60430.jpg)
- 0
- 0
Reactivity 정보
제품 세부 정보
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
Knockout 검증
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
품질 관리
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
초기 취급 지침
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability. <p>1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.<br>2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.<br>3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.<br>4. Incubate the culture at 37°C incubator with 5% CO<sub>2</sub>. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.<br>5. Once confluent passage into an appropriate flask at a density of 2x10<sup>4</sup> cells/cm<sup>2</sup>. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.</p>
Subculture 가이드라인
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
배양 배지
DMEM (High Glucose) + 10% FBS
동결보존 배지
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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