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AB306712

Human ITPR1 knockout U-87 MG cell line

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ITPR1 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.

대체 명칭 보기

ITPR1, Type 1 InsP3 receptor, INSP3R1, InsP3R1, IP3R 1, IP3 receptor isoform 1

1 이미지
Next Generation Sequencing - Human ITPR1 knockout U-87 MG cell line (AB306712)
  • NGS

Lab

Next Generation Sequencing - Human ITPR1 knockout U-87 MG cell line (AB306712)

1 bp insertion and 110 bp deletion in exon 19, CCDS46740.2

주요 정보

세포 유형

U-87 MG

Species or organism

Human

조직

Brain

제형

Liquid

form

Knockout 검증

Next Generation Sequencing

질병

Glioblastoma

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보완 제품

Primary Antibodies

AB282273

Anti-Psoriasin antibody [EPR23482-210] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Psoriasin antibody [EPR23482-210] - BSA and Azide free (Detector) (AB282273)

  • 0
  • 0
Assay Kits

AB64029

Mouse Interferon gamma ELISPOT Kit

  • 0
  • 0
Assay Kits

AB277397

Human Nrf2 ELISA Kit

Sandwich ELISA - Human Nrf2 ELISA Kit (AB277397)

  • 0
  • 0
Assay Kits

AB314384

Human S100A7 ELISA Kit

Sandwich ELISA - Human S100A7 ELISA Kit (AB314384)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "NGS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
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제품 구성

{ "values": { "2x1000000Cellsvial": { "sellingSize": "2 x 1000000 Cells/vial", "publicAssetCode":"ab306712-2x1000000Cells_vial", "assetComponentDetails": [ { "size":"1 x 1000000 Cells/vial", "name":"ab279979 Human wild-type U-87 MG cell line", "number":"AB306712-CMP02" }, { "size":"1 x 1000000 Cells/vial", "name":"ab306712 Human ITPR1 knockout U-87 MG cell line", "number":"AB306712-CMP01" } ] }, "1000000Cellsvial": { "sellingSize": "1000000 Cells/vial", "publicAssetCode":"ab306712-1000000Cells_vial", "assetComponentDetails": [ { "size":"1 x 1000000 Cells/vial", "name":"ab306712 Human ITPR1 knockout U-87 MG cell line", "number":"AB306712-CMP01", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
ITPR1
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Next Generation Sequencing
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-196°C
적절한 장기 보관 조건
-196°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The IP3 receptor (IP3R) also known as inositol 145-trisphosphate receptor functions as a ligand-gated calcium channel. It plays a major role in calcium release from the endoplasmic reticulum into the cytoplasm. The IP3 receptor has a molecular mass of approximately 313 kDa. IP3 receptor expression is extensive found in various tissues including the brain liver and smooth muscle cells. It is an essential component of intracellular calcium signaling mechanisms directly activated by IP3 a secondary messenger molecule.
Biological function summary

IP3 receptor involves in various cellular processes by mediating intracellular calcium release. Upon binding of IP3 produced by phospholipase C catalysis of PIP2 the channel opens to allow calcium ions to flow into the cytosol. The IP3 receptor forms a tetrameric complex essential for its function. This complex is vital for responses in signal transduction pathways affecting T-cell activation muscle contraction and neurotransmitter release.

Pathways

IP3 receptor is central to calcium signaling and interacts with the IP3 pathway modulating cellular activities in response to different stimuli. The interaction with proteins such as TRPC3 a transient receptor potential channel integrates calcium influx. IP3 receptor also participates in the calcium/calmodulin-dependent protein kinase (CaMK) pathway impacting phosphorylation events in the cell.

Dysregulation of IP3 receptor function associates with neurodegenerative diseases such as Alzheimer's Disease due to calcium homeostasis disruption. The receptor also connects to heart diseases where abnormal calcium signaling can lead to arrhythmic conditions. The interaction between the IP3 receptor and other proteins like ryanodine receptors may exacerbate these conditions influencing cellular calcium dynamics in pathological states.
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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

초기 취급 지침

<ul> <li>All seeding densities should be based on cell counts gained by established methods.</li> <li>A guide seeding density of 2x10<sup>4</sup> cells/cm<sup>2</sup> is recommended.</li> <li>Cells should be passaged when they have achieved 80-90% confluence.</li> </ul>

Subculture 가이드라인
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
배양 배지

EMEM + 10% FBS

동결보존 배지

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

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제품 프로토콜

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타겟 정보

See full target information ITPR1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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