AB265930

Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell line

Advanced Validation
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NPR1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 5 bp deletion in exon 1. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.

대체 명칭 보기

ANPRA, Guanylate cyclase A, NPR-A, ANP-A, NPR1, Atrial natriuretic peptide receptor type A, Atrial natriuretic peptide receptor 1, ANPR-A, GC-A

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Lab

Western blot - Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell line (AB265930)

Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : NPR1 knockout HeLa cell lysate 20 μg
Lane 3 : U-251 MG cell lysate 20 μg
Lane 4 : Human Heart tissue lysate 20 μg

Lanes 1 - 4 : Merged signal (red and green). Green - anti-NPR1 antibody observed at 140 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
anti-NPR1 antibody was shown to react with NPR1 in wild-type HeLa cells in Western blot with loss of signal observed in NPR1 knockout cell line ab265930 (NPR1 knockout cell lysate ab258080). Wild-type HeLa and NPR1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with anti-NPR1 antibody and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4^°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

anti-NPR1 antibody at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell lysate (<a href='/ko/products/cell-lysates/human-npr1-natriuretic-peptide-receptor-a-gc-a-knockout-hela-cell-lysate-ab258080'>ab258080</a>) at 20 µg

Lane 3:

U-251 MG cell lysate at 20 µg

Lane 4:

Human Heart tissue lysate at 20 µg

Secondary

Lanes 1 - 3:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Lanes 1 - 3:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

false

Sanger Sequencing - Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell line (AB265930)

Sanger seq

Unknown

Sanger Sequencing - Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell line (AB265930)

Allele-1 : 5 bp deletion in exon 1.

Sanger seq

Unknown

Sanger Sequencing - Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell line (AB265930)

Allele-2 : 1 bp insertion in exon 1.

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

제형

Liquid

form

Knockout 검증

Sanger Sequencing,Western blot

Mutation 정보

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 5 bp deletion in exon 1

질병

Adenocarcinoma

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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제품 세부 정보

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "2x1000000Cellsvial": { "sellingSize": "2 x 1000000 Cells/vial", "publicAssetCode":"ab265930-2x1000000Cells_vial", "assetComponentDetails": [ { "size":"1 x 1000000 Cells/vial", "name":"ab255928 Human wild-type HeLa cell line", "number":"AB265930-CMP02" }, { "size":"1 x 1000000 Cells/vial", "name":"ab265930 Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell line", "number":"AB265930-CMP01" } ] }, "2x1000000Cellsvial": { "sellingSize": "2 x 1000000 Cells/vial", "publicAssetCode":"ab265930-2x1000000Cells_vial", "assetComponentDetails": [ { "size":"1 x 1000000 Cells/vial", "name":"ab255928 Human wild-type HeLa cell line", "number":"AB265930-CMP02" }, { "size":"1 x 1000000 Cells/vial", "name":"ab265930 Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell line", "number":"AB265930-CMP01" } ] }, "1000000Cellsvial": { "sellingSize": "1000000 Cells/vial", "publicAssetCode":"ab265930-1000000Cells_vial", "assetComponentDetails": [ { "size":"1 x 1000000 Cells/vial", "name":"ab265930 Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell line", "number":"AB265930-CMP01" } ] }, "1000000Cellsvial": { "sellingSize": "1000000 Cells/vial", "publicAssetCode":"ab265930-1000000Cells_vial", "assetComponentDetails": [ { "size":"1 x 1000000 Cells/vial", "name":"ab265930 Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell line", "number":"AB265930-CMP01", "productcode":"" } ] } } }

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특성 및 보관 정보

유전자명

NPR1

유전자 편집 종류

Knockout

유전자 편집 방식

CRISPR technology

Knockout 검증

Sanger Sequencing, Western blot

배송 시 보관 조건

Dry Ice

적절한 단기 보관 조건

-196°C

적절한 장기 보관 조건

-196°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NPR-A also known as natriuretic peptide receptor A is a guanylyl cyclase-linked receptor with a mass of approximately 120 kDa. It largely expresses in the kidney heart adrenal gland and vascular smooth muscle cells. NPR-A is structured to bind specifically with atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP) facilitating signal transduction. This receptor generates cyclic GMP (cGMP) upon activation which acts as a secondary messenger in various cellular processes.

Biological function summary

Natriuretic peptide receptor A plays a significant role in cardiovascular homeostasis. NPR-A is not part of a larger complex but works closely with other natriuretic peptide systems to regulate blood pressure electrolyte balance and fluid homeostasis. Its activation results in diuresis natriuresis and vasodilation contributing to its function in maintaining cardiovascular health. Efficient functioning of NPR-A is essential for modulating cardiovascular responses.

Pathways

NPR-A is important in the cyclic GMP pathway and the ANP signaling pathway. It interacts with proteins like NPR-C and soluble guanylyl cyclase modulating key physiological responses. NPR-A impacts smooth muscle relaxation and renal function by influencing cGMP levels and related downstream pathways. Its role within these pathways is fundamental to maintaining vascular tone and sodium excretion.

NPR-A associates prominently with cardiovascular diseases such as hypertension and heart failure. In hypertension NPR-A dysregulation can impair proper sodium and water excretion exacerbating the condition. Heart failure involves altered signaling of NPR-A impacting cardiac and renal function. Protein interactions include altered expression of NPR-C affecting natriuretic peptide clearance and disease progression. Understanding NPR-A's function and regulation provides insights into its relationship with cardiovascular pathophysiology and potential therapeutic approaches.

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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

초기 취급 지침

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability. 1. Thaw the vial in 37°C water bath for approximately 1-2 minutes. 2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution. 3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25. 4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed. 5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture 가이드라인

All seeding densities should be based on cell counts gained by established methods.
A guide seeding density of 2x10⁴ cells/cm² is recommended.
Cells should be passaged when they have achieved 80-90% confluence.

배양 배지

DMEM (High Glucose) + 10% FBS

동결보존 배지

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information NPR1

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell line

Western blot - Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell line (AB265930)

Sanger Sequencing - Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell line (AB265930)

Sanger Sequencing - Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell line (AB265930)

주요 정보

세포 유형

Species or organism

조직

제형

Knockout 검증

Mutation 정보

질병

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

제품 구성

특성 및 보관 정보

유전자명

유전자 편집 종류

유전자 편집 방식

Knockout 검증

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

품질 관리

STR analysis

Cell culture

Biosafety level

Adherent/suspension

Gender

초기 취급 지침

Subculture 가이드라인

배양 배지

동결보존 배지

제품 프로토콜

타겟 정보

Product promise