Human PPM1F (POPX2) knockout HEK-293T cell line
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PPM1F KO cell line available to order. KO validated. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
대체 명칭 보기
Protein fem-2 homolog, KIAA0015, Protein phosphatase 1F, Ca(2+)/calmodulin-dependent protein kinase phosphatase, CaMKPase, CaM-kinase phosphatase, PPM1F, Partner of PIX 2, POPX2, hFem-2
- Sanger seq
Unknown
Sanger Sequencing - Human PPM1F (POPX2) knockout HEK-293T cell line (AB266176)
Allele-2 : 1 bp insertion in exon 2.
- Sanger seq
Unknown
Sanger Sequencing - Human PPM1F (POPX2) knockout HEK-293T cell line (AB266176)
Allele-1 : 1 bp insertion in exon2
보완 제품
제품 세부 정보
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
Knockout 검증
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein phosphatase is instrumental in modulating signaling events related to cytoskeletal dynamics and stress responses. PPM1F does not operate in isolation but rather interacts with other proteins to form complexes that fine-tune its activity and stability. For instance PPM1F associates with components of the actin cytoskeleton and stress-related kinases influencing cellular movement and survival responses. Its ability to deactivate key kinases highlights its biological importance in maintaining cellular function.
Pathways
Recent research places PPM1F in mechanistic contexts related to the MAPK signaling and apoptosis pathways. It interacts closely with proteins like MAPKs and CAMKII to mediate responses to external stimuli influencing cellular proliferation and stress outcomes. The modulation of these pathways by PPM1F highlights its role in facilitating the balance between cell survival and apoptotic signaling. This phosphatase therefore has a significant regulatory function in cellular signaling networks.
품질 관리
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
초기 취급 지침
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability. <p>1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.<br>2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.<br>3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.<br>4. Incubate the culture at 37°C incubator with 5% CO<sub>2</sub>. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.<br>5. Once confluent passage into an appropriate flask at a density of 2x10<sup>4</sup> cells/cm<sup>2</sup>. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.</p>
Subculture 가이드라인
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
배양 배지
DMEM (High Glucose) + 10% FBS
동결보존 배지
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com