Human PSEN1 knockout PC-3 cell line

• WB

Lab

Western blot - Human PSEN1 knockout PC-3 cell line (AB290614)

Western blot : Anti-PSEN1 antibody [EP2000Y] (ab76083) staining at 1/2500 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab76083 was shown to bind specifically to PSEN1. A band was observed at 18 kDa in wild-type PC-3 cell lysates with no signal observed at this size in PSEN1 knockout cell line. To generate this image, wild-type and PSEN1 knockout PC-3 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Presenilin 1/PS-1 antibody [EP2000Y] (<a href='/ko/products/primary-antibodies/presenilin-1-ps-1-antibody-ep2000y-ab76083'>ab76083</a>) at 1/2500 dilution

Lane 1:

Wild-type PC-3 cell lysate at 20 µg

Lane 2:

PSEN1 knockout PC-3 cell lysate at 20 µg

Lane 3:

Wild-type HAP1 cell lysate at 20 µg

Lane 4:

PSEN1 knockout HAP1 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

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• WB

Lab

Western blot - Human PSEN1 knockout PC-3 cell line (AB290614)

Western blot : Anti-PSEN1 antibody [5E12] (ab252856) staining at 1/1000 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab252856 was shown to bind specifically to PSEN1. A band was observed at 100 kDa in wild-type PC-3 cell lysates with no signal observed at this size in PSEN1 knockout cell line. To generate this image, wild-type and PSEN1 knockout PC-3 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rat IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Presenilin 1/PS-1 antibody [5E12] (<a href='/ko/products/primary-antibodies/presenilin-1-ps-1-antibody-5e12-ab252856'>ab252856</a>) at 1/1000 dilution

Lane 1:

Wild-type PC-3 cell lysate at 20 µg

Lane 2:

PSEN1 knockout PC-3 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rat IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

Observed band size: 100 kDa

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• NGS

Supplier Data

Next Generation Sequencing - Human PSEN1 knockout PC-3 cell line (AB290614)

104 bp deletion after Tyr188 (allele 1) and 1 bp deletion after Tyr188 of the WT protein (allele 2).