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AB266663

Human RBM3 knockout HEK-293T cell line

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RBM3 KO cell line available to order. KO validated. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 2. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.

대체 명칭 보기

RBM3, RNA-binding motif protein 3, RNA-binding protein 3, RNPL

3 이미지
Western blot - Human RBM3 knockout HEK-293T cell line (AB266663)
  • WB

Lab

Western blot - Human RBM3 knockout HEK-293T cell line (AB266663)

Lanes 1 - 4 : Merged signal (red and green). Green - ab134946 observed at 18 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.

ab134946 was shown to react with RBM3 in wild-type HEK-293T cells in Western blot with loss of signal observed in RBM3 knockout cell line ab266663 (RBM3 knockout cell lysate ab258170). Wild-type HEK-293T and RBM3 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab134946 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 ° at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-RBM3 antibody [EPR6061(2)] (<a href='/ko/products/primary-antibodies/rbm3-antibody-epr60612-ab134946'>ab134946</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

RBM3 Knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human RBM3 knockout HEK-293T cell line (ab266663)

Lane 3:

Hela cell lysate at 20 µg

Lane 4:

LnCap cell lysate at 20 µg

Predicted band size: 17 kDa

Observed band size: 18 kDa

false

Western blot - Human RBM3 knockout HEK-293T cell line (AB266663)
  • WB

Lab

Western blot - Human RBM3 knockout HEK-293T cell line (AB266663)

All lanes:

Western blot - Anti-RBM3 antibody [EPR6061(2)] (<a href='/ko/products/primary-antibodies/rbm3-antibody-epr60612-ab134946'>ab134946</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa ab271142 at 20 µg

Lane 2:

RBM3 knockout HeLa ab274940 at 20 µg

Lane 3:

Wild-type HEK-293T at 20 µg

Lane 4:

RBM3 knockout HEK-293T at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 17 kDa

Observed band size: 18 kDa

false

Sanger Sequencing - Human RBM3 knockout HEK-293T cell line (AB266663)
  • Sanger seq

Unknown

Sanger Sequencing - Human RBM3 knockout HEK-293T cell line (AB266663)

Homozygous : 2 bp deletion in exon 2

주요 정보

세포 유형

HEK-293T

Species or organism

Human

조직

Kidney

제형

Liquid

form

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 2

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보완 제품

Cell Lines & Lysates

AB255377

Human CDH2 (N Cadherin) knockout HEK-293T cell line

Advanced Validation

Immunocytochemistry/ Immunofluorescence - Human CDH2 (N Cadherin) knockout HEK-293T cell line (AB255377)

  • 0
  • 0
Primary Antibodies

AB136333

PE Anti-DC-SIGN antibody [UW60.1]

Flow Cytometry - PE Anti-DC-SIGN antibody [UW60.1] (AB136333)

  • 0
  • 0
Primary Antibodies

AB283256

Alexa Fluor® 488 Anti-KIR3DL1 antibody [EPR24357-139]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-KIR3DL1 antibody [EPR24357-139] (AB283256)

  • 0
  • 0
Cell Lines & Lysates

AB265519

Human SP1 knockout HeLa cell line

Advanced Validation

ChIP-sequencing - Human SP1 knockout HeLa cell line (AB265519)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Sanger seq": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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제품 구성

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특성 및 보관 정보

유전자명
RBM3
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing
Zygosity
Homozygous
배송 시 보관 조건
Dry Ice
적절한 단기 보관 조건
-196°C
적절한 장기 보관 조건
-196°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

RBM3 also known as RNA-binding motif protein 3 is a cold-inducible protein with a mass of approximately 17 kDa. It plays a role in binding RNA and is widely expressed in tissues including the brain heart and skeletal muscle. Research often highlights its involvement in temperature response implicating its role in cellular adaptation to cold stress. Overexpression of RBM3 has been documented during hypothermic conditions which suggests an adaptive function in extreme environments.
Biological function summary

RBM3 functions by modulating RNA stability and translation impacting cellular stress responses. It forms part of ribonucleoprotein complexes influencing the processing of several RNA species. The protein enhances mRNA stability under stress conditions and promotes cell survival by impacting protein synthesis. RBM3 also participates in synaptic plasticity which is essential for learning and memory showcasing its extensive influence on cellular functioning.

Pathways

RBM3 significantly contributes to neuroprotection and anti-apoptosis pathways. It is closely linked to pathways dealing with stress granules and mRNA stabilization. A known association exists between RBM3 and proteins such as CIRP (cold-inducible RNA-binding protein) which share similar pathways concerning mRNA regulation under cold conditions. This connection highlights RBM3's role in cell survival and stress management.

RBM3 has shown connections to neurodegenerative diseases and cancer. High levels of RBM3 expression correlate with improved outcomes in hypothermia-treated stroke and trauma patients due to its neuroprotective abilities. In cancer RBM3 links with proteins such as HIF-1α (hypoxia-inducible factor-1 alpha) where its overexpression has been associated with tumor progression in certain types suggesting RBM3's dual protective and pathological roles depending on the disease context.
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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

초기 취급 지침

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability. <p>1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.<br>2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.<br>3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.<br>4. Incubate the culture at 37°C incubator with 5% CO<sub>2</sub>. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.<br>5. Once confluent passage into an appropriate flask at a density of 2x10<sup>4</sup> cells/cm<sup>2</sup>. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.</p>

Subculture 가이드라인
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
배양 배지

DMEM (High Glucose) + 10% FBS

동결보존 배지

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

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제품 프로토콜

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타겟 정보

See full target information RBM3
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Product promise

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