Human SLC25A22 (GC-1) knockout HEK-293T cell line
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- Sanger seq
Unknown
Sanger Sequencing - Human SLC25A22 (GC-1) knockout HEK-293T cell line (AB266327)
Allele-1 : 8 bp deletion in exon 3
- Sanger seq
Lab
Sanger Sequencing - Human SLC25A22 (GC-1) knockout HEK-293T cell line (AB266327)
Sequencing chromatogram displaying sequence edit in exon 3
- Sanger seq
Unknown
Sanger Sequencing - Human SLC25A22 (GC-1) knockout HEK-293T cell line (AB266327)
Allele-2 : 1 bp insertion in exon 3.
보완 제품
제품 세부 정보
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
Knockout 검증
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GC-1 plays an important role in the signal transduction pathways involving nitric oxide as part of a complex with protein partners. As a subunit of the soluble guanylate cyclase (sGC) complex it helps mediate the effects of nitric oxide by regulating cGMP levels. This activity is essential for promoting vasodilation and blood flow which is observed in the cardiovascular system. The GC-1 complex translates extracellular signals to intracellular responses affecting various physiological processes.
Pathways
GC-1 is a critical component in the nitric oxide signaling pathway where it facilitates communication between nitric oxide and cGMP-dependent pathways. It interacts closely with proteins such as endothelial nitric oxide synthase (eNOS) and is directly involved in the pathway regulating vascular tone and blood pressure. Additionally GC-1 is part of the phototransduction pathway in retinal cells influencing the conversion of light into electrical signals through its production of cGMP.
품질 관리
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
초기 취급 지침
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability. <p>1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.<br>2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.<br>3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.<br>4. Incubate the culture at 37°C incubator with 5% CO<sub>2</sub>. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.<br>5. Once confluent passage into an appropriate flask at a density of 2x10<sup>4</sup> cells/cm<sup>2</sup>. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.</p>
Subculture 가이드라인
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
배양 배지
DMEM (High Glucose) + 10% FBS
동결보존 배지
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com