- WB
Collaborator
Western blot - Human TICAM1 knockout U-87MG cell line (AB305279)
ab302562 was shown to react with TICAM1 in wild-type U-87 MG cells in Western blot with loss of signal observed in TICAM1 knockout cell line ab305279. Wild-type U-87 MG and TICAM1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab302562 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.
This data was provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes:
Western blot at 1/1000 dilution
Lane 1:
Wild-type U-87 MG lysate at 30 µg
Lane 2:
Western blot - Human TICAM1 knockout U-87MG cell line (ab305279) at 30 µg
Lane 2:
TICAM1 knock-out U-87 MG lysate at 30 µg
false
- Sanger seq
Supplier Data
Sanger Sequencing - Human TICAM1 knockout U-87MG cell line (AB305279)
Homozygote. 392 bp deletion
보완 제품
Primary Antibodies
AB307646
Anti-KMT6 / EZH2 antibody [EPR25353-284]
BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT6 / EZH2 antibody [EPR25353-284] (AB307646)](https://content.abcam.com/products/images/kmt6-ezh2-antibody-epr25353-284-ab307646--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img406510.jpg)
- 0
- 0
Primary Antibodies
AB108928
Anti-FANCD2 antibody [EPR2302]
20ul selling size|KO Validated|RabMAb|Recombinant
![Flow Cytometry (Intracellular) - Anti-FANCD2 antibody [EPR2302] (AB108928)](https://content.abcam.com/products/images/fancd2-antibody-epr2302-ab108928--flow-cytometry-intracellular-img37350.jpg)
- 5
- 4
Primary Antibodies
AB134154
RabMAb|Recombinant|KO Validated|20ul selling size
![Immunocytochemistry/ Immunofluorescence - Anti-TACC3 antibody [EPR7756] (AB134154)](https://content.abcam.com/products/images/tacc3-antibody-epr7756-ab134154--immunocytochemistry-immunofluorescence-img124253.jpg)
- 0
- 0
Reactivity 정보
제품 세부 정보
Recommended control: Human wild-type U-87 MG cell line (ab278079). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture medium: EMEM + 10% FBS
Initial handling guidelines: Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.
Subculture guidelines:
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- A partial media change 24 hours prior to subculture may be helpful to encourage growth, if required.
- Cells should be passaged when they have achieved 80-90% confluence.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
Knockout 검증
배송 시 보관 조건
적절한 단기 보관 기간
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
TRIF is part of the innate immune response mechanism. It functions as a central component in signal transduction processes especially in response to pathogen-associated molecular patterns (PAMPs). TRIF often forms complexes with other signaling proteins to activate transcription factors such as IRF3 and NF-kB which stimulate the expression of inflammatory cytokines and antiviral genes. This function allows the body to respond quickly to infections and initiate immune defenses.
Pathways
TRIF participates in important immune signaling pathways notably the TLR and interferon signaling pathways. In the TLR pathway TRIF interacts with proteins such as MyD88 and TRAF6 to propagate signals from TLR4 critical in detecting Gram-negative bacteria. Through its role in the interferon signaling pathway TRIF helps in the regulation of antiviral responses and is essential for the expression of interferon-stimulated genes that combat viral infections.
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
초기 취급 지침
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability. <p>1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.<br>2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.<br>3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.<br>4. Incubate the culture at 37°C incubator with 5% CO<sub>2</sub>. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.<br>5. Once confluent passage into an appropriate flask at a density of 2x10<sup>4</sup> cells/cm<sup>2</sup>. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.</p>
Subculture 가이드라인
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
배양 배지
EMEM + 10% FBS
동결보존 배지
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com