ABCF3 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
대체 명칭 보기
ATP-binding cassette sub-family F member 3, ABCF3
- Sanger seq
Unknown
Sanger Sequencing - Human ABCF3 knockout HeLa cell lysate (AB258774)
Homozygous : 1 bp insertion in exon 2
보완 제품
제품 세부 정보
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
Knockout 검증
Zygosity
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ABCF3 plays a role in cellular stress response and protein synthesis regulation. It often forms part of a multi-protein complex aiding the translation process by interacting with ribosomal subunits. This interaction suggests an involvement in the regulation of translation initiation which is important for effective protein synthesis. Additionally ABCF3 may have a role in maintaining cellular homeostasis under stress conditions.
Pathways
The ABCF3 protein significantly contributes to the mechanistic target of rapamycin (mTOR) signaling pathway and the integrated stress response pathway. Its interaction with ribosomal proteins suggests a regulatory effect on mTOR signaling which is integral to cell growth and metabolism. ABCF3 also indirectly influences other signaling proteins involved in these pathways such as eukaryotic initiation factors which are key translational regulators.
품질 관리
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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