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AB258297

Human ADCY3 (AC3) knockout HeLa cell lysate

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ADCY3 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.

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Adenylate cyclase type III, ATP pyrophosphate-lyase 3, AC-III, Adenylyl cyclase 3, KIAA0511, ADCY3, AC3, Adenylate cyclase type 3

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Sanger Sequencing - Human ADCY3 (AC3) knockout HeLa cell lysate (AB258297)
  • Sanger seq

Unknown

Sanger Sequencing - Human ADCY3 (AC3) knockout HeLa cell lysate (AB258297)

Homozygous : 1 bp deletion in exon1

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.

질병

Adenocarcinoma

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보완 제품

Primary Antibodies

AB192985

Anti-Histone H3 (tri methyl K27) antibody [EPR18607] - ChIP Grade

20ul selling size|RabMAb|Advanced Validation|Recombinant

ChIP-sequencing - Anti-Histone H3 (tri methyl K27) antibody [EPR18607] - ChIP Grade (AB192985)

  • 4
  • 14
Primary Antibodies

AB4729

Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729)

  • 5
  • 85
Primary Antibodies

AB8898

Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (AB8898)

  • 5
  • 92
Primary Antibodies

AB31830

Anti-Histone H4 antibody [mAbcam 31830] - ChIP Grade

BOND RX™ Validated

Western blot - Anti-Histone H4 antibody [mAbcam 31830] - ChIP Grade (AB31830)

  • 5
  • 6
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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258297-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB258297-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human ADCY3 knockout HeLa cell lysate", "number":"AB258297-CMP01", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
ADCY3
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein AC3 also known as 'Adcy3' functions as an adenylate cyclase enzyme. This enzyme plays a significant mechanical role by converting ATP (adenosine triphosphate) to cyclic AMP (cAMP) which acts as a secondary messenger in various signaling pathways. It is also referred to as '1a12' in some studies. Weighing approximately 137 kDa AC3 is mainly expressed in the olfactory epithelium where it contributes to odor detection the brain and other tissues at lower levels. This protein operates at the cell membrane where it interacts with G proteins to mediate signal transduction processes.
Biological function summary

Adenylate cyclase 3 (AC3) serves as an essential component in sensory signal transduction and is part of the adenylyl cyclase complex. It plays an important role in the conversion of external stimuli into intracellular signals important for processes like olfaction and hormonal response. Within neurons AC3 influences synaptic plasticity and learning by regulating cAMP levels. This modulation affects various neural activities and responses making AC3 important for central nervous system functions.

Pathways

AC3 is integrally involved in the G-protein coupled receptor (GPCR) signaling pathways. These pathways involve the activation of AC3 by G-proteins leading to enhanced production of cAMP which subsequently activates protein kinase A (PKA). AC3 collaborates with other adenylate cyclases and G-protein subunits such as Gα and Gβγ within these pathways. This intricate signaling cascade influences cellular responses to hormones and neurotransmitters and alters gene expression through cAMP-mediated pathways.

AC3 has associations with conditions like obesity and depression. Abnormalities in AC3 function or expression can lead to impaired cAMP signaling disrupting normal metabolic and neurological processes. Research indicates that AC3 interacts with the melanocortin-4 receptor (MC4R) in regulating energy homeostasis and body weight linking it to obesity. In depression altered AC3 levels impact neurotransmitter signaling contributing to mood disorders. Understanding AC3's role in these conditions may offer therapeutic insights into disease management.
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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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제품 프로토콜

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Product promise

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