Human ARRB2 (Beta Arrestin 2) knockout HEK-293T cell lysate
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ARRB2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4.
대체 명칭 보기
Non-visual arrestin-3, ARRB2, Beta-arrestin-2, ARR2, ARB2, Arrestin beta-2
- WB
Unknown
Western blot - Human ARRB2 (Beta Arrestin 2) knockout HEK-293T cell lysate (AB257282)
Lane 1 : Wild-type HEK293T cell lysate (20 μg)
Lane 2 : ARRB2 knockout HEK293T cell lysate (20 μg)
Lane 3 : Mouse brain tissue lysate (20 μg)
Lane 4 : Human lung tissue lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab54790 observed at 48-55 kDa. Red - loading control, ab181602 observed at 37 kDa.
ab54790 Anti-Beta Arrestin 2 antibody was shown to specifically react with Beta Arrestin 2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266116 (knockout cell lysate ab257282) was used. Wild-type and Beta Arrestin 2 knockout samples were subjected to SDS-PAGE. ab54790 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Beta Arrestin 2 antibody [3G1] (<a href='/ko/products/primary-antibodies/beta-arrestin-2-antibody-3g1-ab54790'>ab54790</a>) at 1/500 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
ARRB2 knockout HEK293T cell lysate at 20 µg
Lane 3:
Mouse brain tissue lysate at 20 µg
Lane 4:
Human lung tissue lysate at 20 µg
Predicted band size: 46 kDa
Observed band size: 48-55 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human ARRB2 (Beta Arrestin 2) knockout HEK-293T cell lysate (AB257282)
Homozygous : 1 bp deletion in exon4
- WB
Supplier Data
Western blot - Human ARRB2 (Beta Arrestin 2) knockout HEK-293T cell lysate (AB257282)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
In Western blot, ab314213 was shown to bind specifically to Beta Arrestin 2. A band was observed at 50 kDa and 37kDa in wild-type 293T cell lysates whereas loss of signal was observed in the ARRB2 knockout cell line ab266116 (knockout cell lysate ab257282).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes:
Western blot - Anti-Beta Arrestin 2 antibody [EPR25410-70] (<a href='/ko/products/primary-antibodies/beta-arrestin-2-antibody-epr25410-70-ab314213'>ab314213</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2:
Western blot - Human ARRB2 (Beta Arrestin 2) knockout HEK-293T cell lysate (ab257282) at 20 µg
Lane 3:
HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4:
HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 37 kDa,50 kDa
false
Exposure time: 180s
보완 제품
Reactivity 정보
제품 세부 정보
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
Knockout 검증
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Beta-arrestin 2 plays an important role in mediating signal transduction pathways beyond GPCR desensitization including those involved in cell communication and cellular response regulation. Beta-arrestin 2 interacts with multiple signaling proteins to form complexes that facilitate signaling pathways notably influencing MAPK (Mitogen-Activated Protein Kinase) pathways. It provides a scaffold for downstream signaling molecules affecting cell signaling outcomes.
Pathways
Beta-arrestin 2 significantly participates in two important biological pathways: the MAPK pathway and the Wnt signaling pathway. Through these pathways beta-arrestin 2 interacts with signaling proteins such as ERK1/2 (Extracellular signal-Regulated Kinases) and dishevelled proteins. These interactions highlight beta-arrestin 2's ability to modulate signal strength and duration contributing to diverse cellular responses.
품질 관리
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com