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AB258787

Human ATG2A knockout HeLa cell lysate

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ATG2A KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon13 and 1 bp insertion in exon13 and Insertion of the selection cassette in exon13.

대체 명칭 보기

ATG2A, KIAA0404, Autophagy-related protein 2 homolog A

4 이미지
Sanger Sequencing - Human ATG2A knockout HeLa cell lysate (AB258787)
  • Sanger seq

Unknown

Sanger Sequencing - Human ATG2A knockout HeLa cell lysate (AB258787)

Allele-4 : Insertion of the selection cassette in exon13

Sanger Sequencing - Human ATG2A knockout HeLa cell lysate (AB258787)
  • Sanger seq

Unknown

Sanger Sequencing - Human ATG2A knockout HeLa cell lysate (AB258787)

Allele-3 : Insertion of the selection cassette in exon13

Sanger Sequencing - Human ATG2A knockout HeLa cell lysate (AB258787)
  • Sanger seq

Unknown

Sanger Sequencing - Human ATG2A knockout HeLa cell lysate (AB258787)

Allele-2 : 1 bp insertion in exon13

Sanger Sequencing - Human ATG2A knockout HeLa cell lysate (AB258787)
  • Sanger seq

Unknown

Sanger Sequencing - Human ATG2A knockout HeLa cell lysate (AB258787)

Allele-1 : 1 bp deletion in exon13

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon13 and 1 bp insertion in exon13 and Insertion of the selection cassette in exon13.

질병

Adenocarcinoma

제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

제품 구성

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특성 및 보관 정보

유전자명
ATG2A
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ATG2A also known as Autophagy-related protein 2 homolog A is an important protein involved in autophagy. It has a molecular mass of approximately 230 kDa and is expressed in tissues like the liver heart and skeletal muscle. Mechanically ATG2A is important for the lipid transfer between membranes which is essential for the expansion of autophagosomes cellular structures that engulf and degrade damaged cellular materials.
Biological function summary

ATG2A plays an important role in macroautophagy by forming part of a multiprotein complex called the ATG9 complex. It participates in membrane tethering and expansion during autophagosome formation. This protein interacts with other autophagy-related proteins including the ATG8 family to facilitate the progression of the autophagic process thereby maintaining cellular health and homeostasis.

Pathways

ATG2A operates mainly within the autophagy pathway specifically the PI3K-AKT-mTOR signaling pathway which is vital for cellular survival and growth. In this context ATG2A acts in concert with proteins like ULK1 which helps initiate autophagy. The regulation of autophagic flux via ATG2A is critical for responding to cellular stress and adjusting metabolic activities according to the environmental conditions.

Mutations or dysfunction in ATG2A can impact cancer and neurodegenerative disorders such as Alzheimer's disease. The protein's role in cancer links to its association with protein components like Beclin 1 which regulates autophagic vacuole formation and influences cancer cell survival. In Alzheimer's disease ATG2A interactions with neural proteins can affect the degradation of misfolded proteins which is instrumental in disease progression.

품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

제품 프로토콜

Product promise

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