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AB263103

Human ATP5G2 knockout HEK-293T cell lysate

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ATP5MC2 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
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Sanger Sequencing - Human ATP5G2 knockout HEK-293T cell lysate (AB263103)
  • Sanger seq

Unknown

Sanger Sequencing - Human ATP5G2 knockout HEK-293T cell lysate (AB263103)

Homozygous : 1 bp insertion in exon 2

주요 정보

세포 유형

HEK-293T

Species or organism

Human

조직

Kidney

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.

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보완 제품

Cell Lines & Lysates

AB266394

Human ATP5G2 knockout HEK-293T cell line

Sanger Sequencing - Human ATP5G2 knockout HEK-293T cell line (AB266394)

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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab263103-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human ATP5G2 knockout HEK293T cell lysate", "number":"AB263103-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB263103-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
ATP5MC2
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing
Zygosity
Homozygous
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ATP5G2 also known as ATP synthase subunit c mitochondrial 2 plays a mechanical role in the production of ATP. This protein functions as a component of the Fo subunit of ATP synthase which operates in the mitochondrial membrane. ATP5G2 has a mass of approximately 8.2 kDa and appears in tissues with high energy demand like muscle and nerve cells. The protein's primary role is to facilitate proton translocation across the mitochondrial membrane important for ATP synthesis.
Biological function summary

ATP5G2 contributes to the generation of cellular energy by being part of the ATP synthase complex also called Complex V of the mitochondrial electron transport chain. This complex performs oxidative phosphorylation converting ADP and inorganic phosphate into ATP which fuels various cellular processes. The subunit's function within the complex directly impacts cellular energy yields and efficiency. Its interaction with other ATP synthase subunits is vital for maintaining the function of the entire complex.

Pathways

ATP5G2 integrates into the oxidative phosphorylation pathway which is central to energy metabolism. This pathway is important for the conversion of nutrients into usable energy. ATP5G2 works closely with the NADH dehydrogenase complex (Complex I) to enable effective electron transport and subsequent ATP generation. The intricate coordination between these complexes is necessary for the maintenance of the mitochondrial membrane potential and overall cellular energetics.

Mutations or dysfunction in ATP5G2 can link to mitochondrial disorders such as Leigh syndrome which involves neurodegeneration and energy deficits. The protein's malfunction can disrupt ATP synthesis affecting the energy balance in cells particularly those requiring high ATP levels. Additionally ATP5G2 might contribute to metabolic disorders like diabetes where insulin secretion and action depend on proper energy metabolism. The role of ATP5G2 alongside other ATP synthase subunits highlights its importance in maintaining mitochondrial and cellular function.
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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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제품 프로토콜

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Product promise

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