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AB263134

Human CALM2 knockout HeLa cell lysate

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CALM2 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 271 bp deletion in exon 2.
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Sanger Sequencing - Human CALM2 knockout HeLa cell lysate (AB263134)
  • Sanger seq

Unknown

Sanger Sequencing - Human CALM2 knockout HeLa cell lysate (AB263134)

Homozygous : 271 bp deletion in exon2

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, Homozygous: 271 bp deletion in exon 2

질병

Adenocarcinoma

제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

제품 구성

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특성 및 보관 정보

유전자명
CALM2
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing
Zygosity
Homozygous
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Calmodulin 2 (CALM2) is a calcium-binding messenger protein involved in many cellular processes. It is also known as CALM and shows a molecular weight of approximately 16.7 kDa. CALM2 interacts with various protein targets to regulate intracellular calcium levels. This protein is ubiquitous meaning it is expressed in many tissues throughout the body which reflects its essential role in cellular functions.
Biological function summary

CALM2 acts as a critical mediator in calcium signaling pathways. It forms complexes with other proteins to transmit calcium signals that are important for activities such as muscle contraction cell division and memory formation. CALM2 activates various enzymes ion channels and other proteins through its calcium-binding ability thereby translating calcium ion fluctuations into cellular responses.

Pathways

The involvement of CALM2 in calcium-dependent pathways like the calcium signaling pathway and the MAPK/ERK pathway is significant. It interacts with proteins such as calcineurin and kinases like calcium/calmodulin-dependent protein kinase (CaMK) facilitating the transmission of signals inside cells. Its role in these pathways highlights its importance in regulating diverse cellular activities including cell proliferation and gene expression.

CALM2's dysregulation or mutation links to conditions such as cardiac arrhythmia and Alzheimer's disease. Mutations in the CALM2 protein can affect other calcium-binding proteins such as troponin in the context of cardiac diseases leading to abnormal heart rhythms. Additionally its altered activity in neurons associates with Alzheimer's disease indicating a connection to tau protein malfunctions and calcium dysregulation in neurodegenerative disorders.

품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

제품 프로토콜

Product promise

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