AB257203

Human CBS knockout HeLa cell lysate

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KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 5 and 1 bp insertion in exon 5.

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AI047524, AI303044, Beta-thionase, CBS_HUMAN, Cbs cystathionine beta-synthase, Cystathionine beta-synthase, EC 4.2.1.22, HIP 4, MGC18856, MGC18895, MGC37300, Methylcysteine synthase, OTTHUMP00000109416, OTTHUMP00000109418, Serine sulfhydrase

4 이미지

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Western blot - Human CBS knockout HeLa cell lysate (AB257203)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : CBS knockout HeLa cell lysate (20 μg)

Lane 3 : Raji cell lysate (20 μg)

Lanes 1-3 : Merged signal (red and green). Green - ab131155 observed at 70 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab131155 Anti-CBS antibody [EPR8578] was shown to specifically react with CBS in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264950 (knockout cell lysate ab257203) was used. Wild-type and CBS knockout samples were subjected to SDS-PAGE. ab131155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CBS antibody [EPR8578] (<a href='/ko/products/primary-antibodies/cbs-antibody-epr8578-ab131155'>ab131155</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CBS knockout HeLa cell lysate at 20 µg

Lane 3:

Raji cell lysate at 20 µg

Predicted band size: 61 kDa

Observed band size: 70 kDa

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Lab

Western blot - Human CBS knockout HeLa cell lysate (AB257203)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : CBS knockout HeLa cell lysate (20 μg)

Lane 3 : Raji cell lysate (20 μg)

Lanes 1-3 : Merged signal (red and green). Green - ab140600 observed at 70 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab140600 Anti-CBS antibody [EPR8579] was shown to specifically react with CBS in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264950 (knockout cell lysate ab257203) was used. Wild-type and CBS knockout samples were subjected to SDS-PAGE. ab140600 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CBS antibody [EPR8579] (<a href='/ko/products/primary-antibodies/cbs-antibody-epr8579-ab140600'>ab140600</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CBS knockout HeLa cell lysate at 20 µg

Lane 3:

Raji cell lysate at 20 µg

Predicted band size: 61 kDa

Observed band size: 70 kDa

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Sanger Sequencing - Human CBS knockout HeLa cell lysate (AB257203)

Sanger seq

Unknown

Sanger Sequencing - Human CBS knockout HeLa cell lysate (AB257203)

Allele-2 : 1 bp insertion in exon 5

Sanger seq

Unknown

Sanger Sequencing - Human CBS knockout HeLa cell lysate (AB257203)

Allele-1 : 11 bp deletion in exon 5

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

Knockout 검증

Sanger Sequencing,Western blot

Mutation 정보

Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 5 and 1 bp insertion in exon 5.

질병

Adenocarcinoma

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257203-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB257203-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human CBS knockout HeLa cell lysate", "number":"AB257203-CMP02", "productcode":"" } ] } } }

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특성 및 보관 정보

유전자 편집 종류

Knockout

유전자 편집 방식

CRISPR technology

Knockout 검증

Sanger Sequencing, Western blot

배송 시 보관 조건

Ambient - Can Ship with Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Cystathionine beta-synthase (CBS) is an enzyme that catalyzes the conversion of homocysteine and serine into cystathionine. It is sometimes referred to as CBS monelyne or cystathionine synthase. CBS is a heme-containing protein with a molecular mass of approximately 63 kDa. It is expressed widely in tissues including the liver brain and kidney. The activity of CBS is regulated by various factors including the availability of cofactors like pyridoxal 5'-phosphate and adenosine triphosphate (ATP).

Biological function summary

The enzyme CBS plays a role in the transsulfuration pathway where it helps in the metabolism of homocysteine. CBS is a part of a larger complex in some tissues where it interacts with other enzymes involved in sulfur amino acid metabolism. This function is important in maintaining cellular sulfur amino acid balance and protecting cells from oxidative stress caused by elevated levels of homocysteine.

Pathways

CBS functions in the transsulfuration pathway which connects the methionine cycle and the synthesis of glutathione. This pathway is essential for detoxification and antioxidant protection. CBS interrelates with other proteins like cystathionine gamma-lyase (CGL) within this pathway. The interplay between CBS and CGL ensures the conversion of homocysteine to cysteine which is a precursor for the synthesis of glutathione.

CBS deficiency is linked to homocystinuria a disorder characterized by high levels of homocysteine in the blood and urine. Symptoms include cardiovascular problems and developmental delays. Furthermore alterations in CBS activity relate to cardiovascular diseases due to hyperhomocysteinemia. Within these conditions CBS interacts with other proteins involved in homocysteine metabolism such as methionine synthase which also contributes to the complexity of these disorders.

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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human CBS knockout HeLa cell lysate

Western blot - Human CBS knockout HeLa cell lysate (AB257203)

Western blot - Human CBS knockout HeLa cell lysate (AB257203)

Sanger Sequencing - Human CBS knockout HeLa cell lysate (AB257203)

Sanger Sequencing - Human CBS knockout HeLa cell lysate (AB257203)

주요 정보

세포 유형

Species or organism

조직

Knockout 검증

Mutation 정보

질병

보완 제품

Primary Antibodies

Cell Lines & Lysates

Cell Lines & Lysates

Primary Antibodies

Reactivity 정보

제품 세부 정보

제품 구성

특성 및 보관 정보

유전자 편집 종류

유전자 편집 방식

Knockout 검증

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

품질 관리

STR analysis

Cell culture

Biosafety level

Adherent/suspension

Gender

제품 프로토콜

Product promise