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AB274984

Human CD1A knockout Jurkat cell lysate

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CD1A KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift: 99%.
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Western blot - Human CD1A knockout Jurkat cell lysate (AB274984)
  • WB

Lab

Western blot - Human CD1A knockout Jurkat cell lysate (AB274984)

Lane 1 : Wild-type Jurkat cell lysate 20 μg
Lane 2 : CD1A knockout Jurkat cell lysate 20 μg
Lane 3 : MOLT-4 cell lysate 20 μg
Lane 4 : HeLa cell lysate 20 μg
False colour image of Western blot : Anti-CD1a antibody [EP3091] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab76531 was shown to bind specifically to CD1a. A band was observed at 45-50 kDa in wild-type Jurkat cell lysates with no signal observed at this size in CD1A knockout cell line ab274926 (knockout cell lysate ab274984). To generate this image, wild-type and CD1A knockout Jurkat cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-CD1a antibody [EP3091] (<a href='/ko/products/primary-antibodies/cd1a-antibody-ep3091-ab76531'>ab76531</a>) at 1/1000 dilution

Lane 1:

Wild-type Jurkat cell lysate at 20 µg

Lane 2:

CD1A knockout Jurkat cell lysate at 20 µg

Lane 2:

Western blot - Human CD1A knockout Jurkat cell line (<a href='/ko/products/cell-lines/human-cd1a-knockout-jurkat-cell-line-ab274926'>ab274926</a>)

Lane 3:

MOLT-4 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L preabsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat anti-Mouse IgG H&L preabsorbed (<a href='/ko/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

Predicted band size: 37 kDa

Observed band size: 45-50 kDa

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Western blot - Human CD1A knockout Jurkat cell lysate (AB274984)
  • WB

Lab

Western blot - Human CD1A knockout Jurkat cell lysate (AB274984)

Lane 1 : Wild-type Jurkat cell lysate 20 μg
Lane 2 : CD1A knockout Jurkat cell lysate 20 μg
Lane 3 : MOLT-4 cell lysate 20 μg
Lane 4 : HeLa cell lysate 20 μg
False colour image of Western blot : Anti-CD1a antibody [EP3622] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab108309 was shown to bind specifically to CD1a. A band was observed at 45-50 kDa in wild-type Jurkat cell lysates with no signal observed at this size in CD1A knockout cell line ab274926 (knockout cell lysate ab274984). To generate this image, wild-type and CD1A knockout Jurkat cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-CD1a antibody [EP3622] (<a href='/ko/products/primary-antibodies/cd1a-antibody-ep3622-ab108309'>ab108309</a>) at 1/1000 dilution

Lane 1:

Wild-type Jurkat cell lysate at 20 µg

Lane 2:

CD1A knockout Jurkat cell lysate at 20 µg

Lane 2:

Western blot - Human CD1A knockout Jurkat cell line (<a href='/ko/products/cell-lines/human-cd1a-knockout-jurkat-cell-line-ab274926'>ab274926</a>)

Lane 3:

MOLT-4 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Predicted band size: 37 kDa

Observed band size: 45-50 kDa

false

주요 정보

세포 유형

Jurkat

Species or organism

Human

조직

Blood

Knockout 검증

Next Generation Sequencing,Western blot

Mutation 정보

Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift: 99%

질병

Non-Hodgkin Lymphoma

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보완 제품

Primary Antibodies

AB108309

Anti-CD1a antibody [EP3622]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1a antibody [EP3622] (AB108309)

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Proteins & Peptides

AB114359

Recombinant Human CD1a protein

SDS-PAGE - Recombinant Human CD1a protein (AB114359)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab274984-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human CD1A knockout Jurkat cell lysate", "number":"AB274984-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type Jurkat cell lysate", "number":"AB274984-CMP01", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
CD1A
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Next Generation Sequencing, Western blot
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CD1a also known as T6 antigen or CD1 is a protein with an estimated molecular mass of 37 kDa. It is expressed mainly on the surface of Langerhans cells in the skin and dendritic cells in the thymus. CD1a belongs to the CD1 family which presents lipid antigens to T cells. The protein is an important marker in immunophenotyping of these dendritic cells making it useful in flow cytometry applications.
Biological function summary

CD1a participates in the immune system by presenting lipid and glycolipid antigens to T lymphocytes including natural killer T (NKT) cells. It forms a complex with β2-microglobulin which is essential for its proper function and antigen presentation. CD1a expression helps differentiate between types of dendritic cells with CD1a-positive cells often engaging in specific responses to microbial lipid antigens.

Pathways

CD1a plays a significant role in the antigen presentation pathway particularly in lipid antigen presentation to T cells. This is important for initiating immune responses. It interacts with related proteins such as CD1b and CD1c which also present lipid antigens. CD1a has implications in innate and adaptive immunity and can influence pathways involved in microbial recognition and elimination.

CD1a is associated with certain skin conditions like Langerhans Cell Histiocytosis and psoriasis. It is involved in the pathogenesis of these diseases by affecting immune responses in the skin. In Langerhans Cell Histiocytosis CD1a-positive cells accumulate abnormally. Additionally CD1a is often studied alongside related proteins like CD207 (Langerin) to understand these conditions better.
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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Suspension

Gender

Male

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제품 프로토콜

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Product promise

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