AB275531

Human CXCR5 knockout Raji cell lysate

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CXCR5 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 19 bp deletion in exon 2.

대체 명칭 보기

Burkitt lymphoma receptor 1, MDR15, MDR-15, C-X-C chemokine receptor type 5, CXCR5, CD185, Monocyte-derived receptor 15, BLR1, CXC-R5, CXCR-5

2 이미지

Lab

Western blot - Human CXCR5 knockout Raji cell lysate (AB275531)

Lane 1 : Wild-type Raji cell lysate 30 ug
Lane 2 : CXCR5 knockout Raji cell lysate 30 ug
Lane 3 : Daudi cell lysate 30 ug
Lane 4 : Jurkat cell lysate 30 ug
Lanes 1 - 4 : Merged signal (red and green). Green - ab254415 observed at 60 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab254415 was shown to react with CXCR5 in wild-type Raji cells in Western blot with loss of signal observed in CXCR5 knockout sample. Wild-type and CXCR5 knockout Raji cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab254415 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 ^°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-CXCR5 antibody [EPR23463-30] (<a href='/ko/products/primary-antibodies/cxcr5-antibody-epr23463-30-ab254415'>ab254415</a>) at 1/1000 dilution

Lane 1:

Wild-type Raji cell lysate at 30 µg

Lane 2:

CXCR5 knockout Raji cell lysate at 30 µg

Lane 2:

Western blot - Human CXCR5 knockout Raji cell line (<a href='/ko/products/cell-lines/human-cxcr5-knockout-raji-cell-line-ab273380'>ab273380</a>)

Lane 3:

Daudi cell lysate at 30 µg

Lane 4:

Jurkat cell lysate at 30 µg

Predicted band size: 42 kDa

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Sanger Sequencing - Human CXCR5 knockout Raji cell lysate (AB275531)

Sanger seq

Supplier Data

Sanger Sequencing - Human CXCR5 knockout Raji cell lysate (AB275531)

Homozygous : 19 bp deletion in exon 2

주요 정보

세포 유형

Raji

Species or organism

Human

조직

Lymphatic

Knockout 검증

Sanger Sequencing,Western blot

Mutation 정보

Knockout achieved by using CRISPR/Cas9, Homozygous: 19 bp deletion in exon 2

질병

Lymphoma

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab275531-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type Raji cell lysate", "number":"AB275531-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human CXCR5 knockout Raji cell lysate", "number":"AB275531-CMP02", "productcode":"" } ] } } }

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특성 및 보관 정보

유전자명

CXCR5

유전자 편집 종류

Knockout

유전자 편집 방식

CRISPR technology

Knockout 검증

Sanger Sequencing, Western blot

Zygosity

Homozygous

배송 시 보관 조건

Ambient - Can Ship with Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

CXCR5 also known as CD185 is a chemokine receptor with a mass of approximately 43 kDa. It is part of the G protein-coupled receptor family and is widely expressed on follicular B helper T (Tfh) cells B cells and a subset of circulating blood cells. CXCR5 is recognized as a marker for Tfh cells which are critical for the function and maturation of B cells within germinal centers. In tissues CXCR5 expression appears prominently in regions where immune cell interactions facilitate immune and inflammatory responses.

Biological function summary

CXCR5 serves a significant role in directing B cells and Tfh cells to lymphoid tissues by interacting with its ligand CXCL13. This interaction involves recruitment and organization of B cells into follicles within secondary lymphoid organs. The presence of CXCR5 allows B cells and Tfh cells to navigate the follicular zones efficiently enhance antigen-specific immune responses and contribute to long-lived humoral immunity. CXCR5 functions as part of a complex that includes signaling pathways influencing cellular movements and location.

Pathways

CXCR5 plays a significant part in the immune system's trafficking pathways particularly within the crosstalk between chemokines and their receptors. CXCR5 expression facilitates the CXC chemokine pathway which important for positioning immune cells within lymphoid organs. Additionally CXCR5 interacts with proteins such as CXCL13 working together to pattern the architecture of germinal centers where affinity maturation and class switching of antibodies occur critical processes in adaptive immunity.

CXCR5 has been implicated in autoimmune diseases and certain lymphomas. For example aberrant CXCR5 expression is linked to the pathogenesis of diseases like systemic lupus erythematosus (SLE) where it might contribute to immune dysregulation and chronic inflammation. In the context of certain lymphomas CXCR5's role in lymphoid organ architecture and cell migration becomes even more relevant. Its expression often parallels the presence of CXCL13 hinting at a network of interactions promoting tumor survival and growth within lymphoid landscapes. Understanding these relationships can offer insight into developing therapeutic strategies potentially involving CXCR5 inhibitors or anti-CXCR5 antibodies to address these diseases.

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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Suspension

Gender

Male

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human CXCR5 knockout Raji cell lysate

Western blot - Human CXCR5 knockout Raji cell lysate (AB275531)

Sanger Sequencing - Human CXCR5 knockout Raji cell lysate (AB275531)

주요 정보

세포 유형

Species or organism

조직

Knockout 검증

Mutation 정보

질병

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Cell Lines & Lysates

Reactivity 정보

제품 세부 정보

제품 구성

특성 및 보관 정보

유전자명

유전자 편집 종류

유전자 편집 방식

Knockout 검증

Zygosity

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

품질 관리

STR analysis

Cell culture

Biosafety level

Adherent/suspension

Gender

제품 프로토콜

Product promise