Human DAXX knockout HeLa cell lysate

• Sanger seq

Unknown

Sanger Sequencing - Human DAXX knockout HeLa cell lysate (AB257408)

Allele-2 : 1 bp insertion in exon5

• Sanger seq

Unknown

Sanger Sequencing - Human DAXX knockout HeLa cell lysate (AB257408)

Allele-1 : 1 bp deletion in exon5

• WB

Lab

Western blot - Human DAXX knockout HeLa cell lysate (AB257408)

Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : DAXX knockout HeLa cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab32140 observed at 100 kDa. Red - loading control ab8245 observed at 37 kDa.
ab32140 Recombinant Anti-Daxx antibody [E94] was shown to specifically react with Daxx in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265233 (knockout cell lysate ab257408) was used. Wild-type and Daxx knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32140 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Daxx antibody [E94] (<a href='/ko/products/primary-antibodies/daxx-antibody-e94-ab32140'>ab32140</a>) at 1/5000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human DAXX knockout HeLa cell lysate (ab257408) at 20 µg

Predicted band size: 81 kDa

Observed band size: 100 kDa

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