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AB258839

Human DCAF12 knockout HeLa cell lysate

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DCAF12 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 4 bp deletion in exon1 and Insertion of the selection cassette in exon1.
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Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (AB258839)
  • Sanger seq

Unknown

Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (AB258839)

Allele-1 : 4 bp deletion in exon1

Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (AB258839)
  • Sanger seq

Unknown

Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (AB258839)

Allele-2 : 1 bp deletion in exon1

Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (AB258839)
  • Sanger seq

Unknown

Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (AB258839)

Allele-3 : Insertion of the selection cassette in exon1

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 4 bp deletion in exon1 and Insertion of the selection cassette in exon1.

질병

Adenocarcinoma

제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

제품 구성

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특성 및 보관 정보

유전자명
DCAF12
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C

품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

제품 프로토콜

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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