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AB257934

Human EEF1B2 knockout HEK-293T cell lysate

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EEF1B2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.

대체 명칭 보기

EEF1B2, Elongation factor 1-beta, EF-1-beta, EEF1B, EF1B

2 이미지
Western blot - Human EEF1B2 knockout HEK-293T cell lysate (AB257934)
  • WB

Unknown

Western blot - Human EEF1B2 knockout HEK-293T cell lysate (AB257934)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : EEF1B2 knockout HEK293T cell lysate (20 ug)
Lane 3 : HeLa cell lysate (20 ug)

ab37725 was shown to specifically react with eEF1B2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266769 (knockout cell lysate ab257934) was used. Wild-type and eEF1B2 knockout samples were subjected to SDS-PAGE. ab37725 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4oC at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

false

Sanger Sequencing - Human EEF1B2 knockout HEK-293T cell lysate (AB257934)
  • Sanger seq

Unknown

Sanger Sequencing - Human EEF1B2 knockout HEK-293T cell lysate (AB257934)

Homozygous : 1 bp deletion in exon 1

주요 정보

세포 유형

HEK-293T

Species or organism

Human

조직

Kidney

Knockout 검증

Sanger Sequencing,Western blot

Mutation 정보

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.

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보완 제품

Primary Antibodies

AB93808

Anti-TFE3 antibody

Western blot - Anti-TFE3 antibody (AB93808)

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  • 1
Cell Lines & Lysates

AB266769

Human EEF1B2 knockout HEK-293T cell line

Advanced Validation

Western blot - Human EEF1B2 knockout HEK-293T cell line (AB266769)

  • 0
  • 0
Primary Antibodies

AB264421

Anti-TFEB antibody [BLR070G]

20ul selling size|Recombinant|KO Validated

Flow Cytometry - Anti-TFEB antibody [BLR070G] (AB264421)

  • 0
  • 0
Primary Antibodies

AB40768

Anti-Raptor antibody [EP539Y]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Raptor antibody [EP539Y] (AB40768)

  • 4
  • 3
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257934-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB257934-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human EEF1B2 knockout HEK293T cell lysate", "number":"AB257934-CMP01", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
EEF1B2
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing, Western blot
Zygosity
Homozygous
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The eukaryotic translation elongation factor 1 beta 2 (eEF1B2) also known as EF1B or EF1-beta plays a role in protein translation. Mechanically eEF1B2 is a subunit of the elongation factor complex that facilitates the exchange of GDP for GTP on eEF1A allowing it to deliver aminoacyl-tRNAs to the ribosome during the elongation phase of translation. The protein has an estimated mass of approximately 24 kDa. It expresses widely throughout different tissues indicating its involvement in general cellular processes.
Biological function summary

EEF1B2 assists in the regulation of protein synthesis by forming a complex with other subunits of the elongation factor including eEF1A and eEF1D. This complex ensures the efficient and accurate delivery of tRNA during the translational elongation phase which is essential for proper cellular function and growth. Although eEF1B2 is often expressed in various tissues its expression levels can vary potentially affecting the rate of protein synthesis in different cellular environments.

Pathways

EEF1B2 integrates into the larger translation pathway a primary component of the cellular protein expression network. It also participates in the amino acid and nucleotide biosynthesis pathways by assisting in creating critical cellular proteins. eEF1B2 closely interacts with eEF1A within these pathways demonstrating its direct involvement in translation and its influence on the production of proteins required for other metabolic processes.

The dysregulation of eEF1B2 expression correlates with certain cancers such as ovarian and colorectal cancers where its abnormal expression may disrupt normal protein synthesis and promote tumor growth. Research suggests that eEF1B2 may interact with oncogenic proteins such as c-Myc impacting cell proliferation and survival mechanisms. Understanding the regulation and function of eEF1B2 in these contexts remains vital for developing targeted therapeutic strategies.
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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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제품 프로토콜

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Product promise

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