- Sanger seq
Unknown
Sanger Sequencing - Human EIF3H knockout HeLa cell lysate (AB258408)
Allele-1 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human EIF3H knockout HeLa cell lysate (AB258408)
Allele-2 : Insertion of the selection cassette in exon1
보완 제품
제품 세부 정보
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
Knockout 검증
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
EIF3H facilitates the recruitment of mRNA to the ribosome by stabilizing the interaction between mRNA and the 40S ribosomal subunit. It functions within the eIF3 complex to assist in ribosomal scanning and the start codon selection. As part of this complex EIF3H helps govern the efficiency of translation initiation impacting protein synthesis rates. These activities are important for cell growth and protein expression regulation underlying the complex's importance in cellular processes.
Pathways
EIF3H plays a role in the mTOR signaling pathway and the MAPK/ERK pathway both extensively involved in regulating cell growth and proliferation. Through these pathways EIF3H interacts with proteins such as eIF4G and ribosomal protein S6 which help coordinate the translation machinery's function and cellular metabolic control. These pathways highlight EIF3H's role in translational control affecting overall cellular function.
품질 관리
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com