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AB257173

Human FANCD2 knockout HeLa cell lysate

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FANCD2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 4 bp deletion in exon 13.
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Western blot - Human FANCD2 knockout HeLa cell lysate (AB257173)
  • WB

Lab

Western blot - Human FANCD2 knockout HeLa cell lysate (AB257173)

Lane 1 : Wild-type HeLa cell lysate (20µg)

Lane 2 : FANCD2 knockout HeLa cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab178705 observed at 166 kDa. Red - loading control ab7291 observed at 50 kDa.

ab178705 Anti-FANCD2 antibody [EPR2279(3)] was shown to specifically react with FANCD2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261743 (knockout cell lysate ab257173) was used. Wild-type and FANCD2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab178705 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FANCD2 antibody [EPR2279(3)] (<a href='/ko/products/primary-antibodies/fancd2-antibody-epr22793-ab178705'>ab178705</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

FANCD2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human FANCD2 knockout HeLa cell line (<a href='/ko/products/cell-lines/human-fancd2-knockout-hela-cell-line-ab261743'>ab261743</a>)

Predicted band size: 166 kDa

Observed band size: 166 kDa

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Western blot - Human FANCD2 knockout HeLa cell lysate (AB257173)
  • WB

Lab

Western blot - Human FANCD2 knockout HeLa cell lysate (AB257173)

Lane 1 : Wild-type HeLa cell lysate (20µg)

Lane 2 : FANCD2 knockout HeLa cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab108928 observed at 166 kDa. Red - loading control ab7291 observed at 50 kDa.

ab108928 Anti-FANCD2 antibody [EPR2302] was shown to specifically react with FANCD2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261743 (knockout cell lysate ab257173) was used. Wild-type and FANCD2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108928 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FANCD2 antibody [EPR2302] (<a href='/ko/products/primary-antibodies/fancd2-antibody-epr2302-ab108928'>ab108928</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

FANCD2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human FANCD2 knockout HeLa cell line (<a href='/ko/products/cell-lines/human-fancd2-knockout-hela-cell-line-ab261743'>ab261743</a>)

Predicted band size: 166 kDa

Observed band size: 166 kDa

false

Sanger Sequencing - Human FANCD2 knockout HeLa cell lysate (AB257173)
  • Sanger seq

Unknown

Sanger Sequencing - Human FANCD2 knockout HeLa cell lysate (AB257173)

Homozygous : 4 bp deletion in exon 13

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

Knockout 검증

Sanger Sequencing,Western blot

Mutation 정보

Knockout achieved by using CRISPR/Cas9, Homozygous: 4 bp deletion in exon 13.

질병

Adenocarcinoma

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보완 제품

Cell Lines & Lysates

AB150035

HeLa whole cell lysate

Western blot - HeLa whole cell lysate (AB150035)

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Primary Antibodies

AB108928

Anti-FANCD2 antibody [EPR2302]

20ul selling size|KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-FANCD2 antibody [EPR2302] (AB108928)

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  • 4
Cell Lines & Lysates

AB166833

Hep G2 whole cell lysate

  • 0
  • 0
Cell Lines & Lysates

AB30124

Raji whole cell lysate

Western blot - Raji whole cell lysate (AB30124)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257173-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human FANCD2 knockout HeLa cell lysate", "number":"AB257173-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB257173-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
FANCD2
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing, Western blot
Zygosity
Homozygous
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

FANCD2 also known as Fanconi anemia group D2 functions mechanically in DNA repair processes. This protein plays a role in the cellular response to DNA damage specifically interstrand crosslinks. FANCD2 has a molecular weight of approximately 164 kDa. Expression of FANCD2 occurs broadly in various tissues but reaches higher levels in cells that undergo rapid division such as hematopoietic cells.
Biological function summary

FANCD2 collaborates with multiple proteins as part of the Fanconi anemia (FA) complex to ensure genomic stability. The FA complex operates by facilitating the repair of DNA interstrand crosslinks that impede replication. FANCD2 becomes activated through monoubiquitination which serves as a signal for recruitment to DNA damage sites. It essentially functions as a coordinator that brings together important components for repair.

Pathways

FANCD2 integrates into the Fanconi anemia pathway and the homologous recombination repair pathway. These pathways are critical for maintaining DNA integrity and preventing chromosomal instability. Within these pathways interactions with proteins such as BRCA1 and BRCA2 reinforce the repair processes. FANCD2's connection to these proteins exemplifies its role in complex mechanisms that preserve genomic fidelity.

FANCD2 links significantly to Fanconi anemia a genetic disorder that causes bone marrow failure and increased cancer susceptibility. Damage or malfunction of FANCD2 can disrupt DNA repair leading to cellular dysfunction seen in this condition. Additionally there is a connection to breast cancer whereby FANCD2 interacts with BRCA2 indicating a shared pathway involved in tumor suppressor functions. These associations underline the importance of FANCD2 in disease pathology and its potential as a therapeutic target.
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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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제품 프로토콜

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Product promise

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