- Sanger seq
Unknown
Sanger Sequencing - Human FBXO11 knockout HeLa cell lysate (AB263199)
Allele-2 : Insertion of the selection cassette in exon 8
- Sanger seq
Unknown
Sanger Sequencing - Human FBXO11 knockout HeLa cell lysate (AB263199)
Allele-1 : 5 bp deletion in exon 8
보완 제품
제품 세부 정보
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
Knockout 검증
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
FBXO11 is involved in protein degradation and regulation of the cell cycle. It serves as an important component of the SCF ubiquitin ligase complex where it binds to specific substrates marking them for proteasomal degradation. FBXO11 plays an important role in cellular processes such as DNA damage response and cell growth regulation. By targeting specific proteins for degradation it regulates protein stability which is important for normal cellular proliferation.
Pathways
FBXO11 is critical in the TGF-beta signaling pathway and the apoptosis pathway. In the TGF-beta signaling pathway FBXO11 regulates the stability of inhibitory SMAD proteins like SMAD7 by targeting them for proteasomal degradation. This regulation impacts cellular responses to TGF-beta influencing processes like cell differentiation and immune responses. In apoptosis FBXO11 interacts with other proteins like BCL2 to influence cell survival and programmed cell death.
품질 관리
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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