AB257950

Human FER knockout HeLa cell lysate

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FER KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon4.

대체 명칭 보기

Feline encephalitis virus-related kinase FER, Tyrosine-protein kinase Fer, p94-Fer, Tyrosine kinase 3, FER, TYK3, Fujinami poultry sarcoma/Feline sarcoma-related protein Fer, Proto-oncogene c-Fer

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Lab

Western blot - Human FER knockout HeLa cell lysate (AB257950)

Lane 1 : Wild-type HeLa cell lysate 20 ug
Lane 2 : FER knockout HeLa cell lysate 20 ug
Lanes 1 - 2 : Merged signal (red and green). Green - ab52479 observed at 100 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab52479 was shown to react with FER in wild-type HeLa cells in Western blot with loss of signal observed in FER knockout cell line ab265226 (FER knockout cell lysate ab257950). Wild-type HeLa and FER knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab52479 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 ^°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-FER antibody [EP1842Y] (<a href='/ko/products/primary-antibodies/fer-antibody-ep1842y-ab52479'>ab52479</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

FER knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human FER knockout HeLa cell line (<a href='/ko/products/cell-lines/human-fer-knockout-hela-cell-line-ab265226'>ab265226</a>)

Predicted band size: 95 kDa

Observed band size: 100 kDa

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Sanger Sequencing - Human FER knockout HeLa cell lysate (AB257950)

Sanger seq

Unknown

Sanger Sequencing - Human FER knockout HeLa cell lysate (AB257950)

Homozygous : 13 bp deletion in exon4

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

Knockout 검증

Sanger Sequencing,Western blot

Mutation 정보

Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon4.

질병

Adenocarcinoma

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257950-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB257950-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human FER knockout HeLa cell lysate", "number":"AB257950-CMP01", "productcode":"" } ] } } }

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특성 및 보관 정보

유전자명

FER

유전자 편집 종류

Knockout

유전자 편집 방식

CRISPR technology

Knockout 검증

Sanger Sequencing, Western blot

배송 시 보관 조건

Ambient - Can Ship with Ice

적절한 단기 보관 조건

-20°C

적절한 장기 보관 조건

-20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The FER protein also known as mouse FER or FER-1 functions as a non-receptor tyrosine kinase within the Fes/Fps subfamily. It has a molecular mass of approximately 94 kDa. FER is expressed across various tissues notably in epithelial cells and is also present in hematopoietic and neural cells. Its kinase activity regulates cell signaling affecting processes like cell proliferation differentiation and survival.

Biological function summary

FER acts as a regulator in signaling pathways essential for cellular communication and structure maintenance. FER interacts within cellular complexes modulating the cytoskeleton and intercellular junctions. It influences actin remodeling impacting cell adhesion and motility. FER's involvement in such activities makes it important for maintaining epithelial integrity and cellular response to external stimuli.

Pathways

FER plays a role in the PI3K/Akt pathway and the MAPK/ERK pathway. These pathways are important for cell growth survival and proliferation. FER interacts with other proteins like STAT3 and JAK2 integrating signals that drive these pathways. By modulating key signaling events FER influences cellular functions and responses to environmental changes.

FER associates with cancer and cardiovascular diseases. Aberrant FER expression and activity have been found in various tumors linking it to cancer progression and metastasis. In cardiovascular disease FER's role in regulating endothelial function connects it to vascular abnormalities. Its interaction with proteins like VEGFR further implicates FER in the pathogenesis of these conditions serving as a potential therapeutic target.

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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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제품 프로토콜

Visit the General protocols
Visit the Troubleshooting

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human FER knockout HeLa cell lysate

Western blot - Human FER knockout HeLa cell lysate (AB257950)

Sanger Sequencing - Human FER knockout HeLa cell lysate (AB257950)

주요 정보

세포 유형

Species or organism

조직

Knockout 검증

Mutation 정보

질병

보완 제품

Cell Lines & Lysates

Primary Antibodies

Primary Antibodies

Cell Lines & Lysates

Reactivity 정보

제품 세부 정보

제품 구성

특성 및 보관 정보

유전자명

유전자 편집 종류

유전자 편집 방식

Knockout 검증

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

추가 정보

Biological function summary

Pathways

품질 관리

STR analysis

Cell culture

Biosafety level

Adherent/suspension

Gender

제품 프로토콜

Product promise