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AB258871

Human FGF13 knockout HEK-293T cell lysate

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FGF13 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4.

대체 명칭 보기

Fibroblast growth factor 13, FGF13, Fibroblast growth factor homologous factor 2, FHF-2, FGF-13, FHF2

2 이미지
Sanger Sequencing - Human FGF13 knockout HEK-293T cell lysate (AB258871)
  • Sanger seq

Unknown

Sanger Sequencing - Human FGF13 knockout HEK-293T cell lysate (AB258871)

Homozygous : 1 bp insertion in exon4

Western blot - Human FGF13 knockout HEK-293T cell lysate (AB258871)
  • WB

Lab

Western blot - Human FGF13 knockout HEK-293T cell lysate (AB258871)

All lanes:

Western blot - Anti-FGF13 antibody [EPR29561-52] (<a href='/ko/products/primary-antibodies/fgf13-antibody-epr29561-52-ab325168'>ab325168</a>)

Lane 1:

Wilde-type HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 80 µg

Lane 2:

Western blot - Human FGF13 knockout HEK-293T cell lysate (ab258871) at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 20 kDa,30 kDa,124 kDa

false

Exposure time: 180s

주요 정보

세포 유형

HEK-293T

Species or organism

Human

조직

Kidney

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4.

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보완 제품

Primary Antibodies

AB203676

Anti-tenomodulin antibody

Western blot - Anti-tenomodulin antibody (AB203676)

  • 4
  • 7
Primary Antibodies

AB51560

Anti-TRPC3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRPC3 antibody (AB51560)

  • 5
  • 2
Primary Antibodies

AB184337

Anti-Lhx2/LH2 antibody [EPR20449]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lhx2/LH2 antibody [EPR20449] (AB184337)

  • 5
  • 6
Cell Lines & Lysates

AB257590

Human PLCG1 (Phospholipase C gamma 1/PLC-gamma-1) knockout HEK-293T cell lysate

Western blot - Human PLCG1 (Phospholipase C gamma 1/PLC-gamma-1) knockout HEK-293T cell lysate (AB257590)

  • 0
  • 0
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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258871-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB258871-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human FGF13 knockout HEK293T cell lysate", "number":"AB258871-CMP01", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
FGF13
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

FGF13 also known as Fibroblast Growth Factor 13 is a member of the FGF family and plays a significant role in cellular processes. It has an approximate molecular mass of 29 kDa. You often find FGF13 expressed in the brain and heart indicating its involvement in the physiology of these organs. FGF13 does not function as a traditional growth factor through receptors but instead operates intracellularly engaging directly with cellular components.
Biological function summary

FGF13 plays important roles in regulating neuronal development and intracellular signaling pathways. It forms complexes with other proteins modulating their activities within cells. FGF13 is instrumental in neuronal outgrowth and synaptic plasticity essential functions for maintaining a healthy nervous system. Researchers identify FGF13 as important for proper neuronal positioning and survival.

Pathways

FGF13 is intricately involved in signaling mechanisms associated with ion channel regulation and mitogen-activated protein kinase (MAPK) pathway. It interacts with proteins such as kinases and other ion channel components to fine-tune cellular responses to extracellular stimuli. FGF13 by modulating these pathways assists in the precise control of cellular growth differentiation and adaptation.

FGF13 presents a significant connection to neurodevelopmental and cardiac disorders. Mutations in FGF13 or its altered expression levels have links to disorders such as epilepsy and cardiac arrhythmia. Through these diseases FGF13 often interacts with proteins like ion channels affecting their function and stability. These interactions highlight the potential of FGF13 as a target for therapeutic interventions in related disorders.
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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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제품 프로토콜

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Product promise

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