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AB258897

Human HENMT1 knockout HeLa cell lysate

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HENMT1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 3.
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Sanger Sequencing - Human HENMT1 knockout HeLa cell lysate (AB258897)
  • Sanger seq

Unknown

Sanger Sequencing - Human HENMT1 knockout HeLa cell lysate (AB258897)

Homozygous : Insertion of the selection cassette in exon 3

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 3.

질병

Adenocarcinoma

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보완 제품

Proteins & Peptides

AB98077

Recombinant Human COMMD1/MURR1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human COMMD1/MURR1 protein (His tag N-Terminus) (AB98077)

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  • 0
Cell Lines & Lysates

AB269667

Human Lgals9 (galectin 9/Gal-9) knockout THP-1 cell lysate

Western blot - Human Lgals9 (galectin 9/Gal-9) knockout THP-1 cell lysate (AB269667)

  • 0
  • 0
Assay Kits

AB238536

Protein Carbonyl ELISA Kit

Indirect ELISA - Protein Carbonyl ELISA Kit (AB238536)

  • 5
  • 1
Cell Lines & Lysates

AB263348

Human SLC12A9 knockout HeLa cell lysate

Sanger Sequencing - Human SLC12A9 knockout HeLa cell lysate (AB263348)

  • 0
  • 0
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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258897-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB258897-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human HENMT1 knockout HeLa cell lysate", "number":"AB258897-CMP01", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
HENMT1
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing
Zygosity
Homozygous
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

HENMT1 also known as HEN1 methyltransferase homolog 1 is an enzyme with a molecular mass of approximately 55 kDa. It is expressed in the germ cells of both mammals and plants. This enzyme belongs to the small RNA methylation family and it plays an important role in the modification of small RNAs specifically piwi-interacting RNAs (piRNAs). HENMT1's primary function is transferring a methyl group to the 3' end of piRNAs protecting them against uridylation and exonucleolytic degradation keeping their integrity and stability necessary for their function.
Biological function summary

The enzyme HENMT1 participates in piRNA biogenesis. These piRNAs are part of a larger ribonucleoprotein complex that includes Piwi proteins which are essential for transposon silencing in the germline. This complex ensures the epigenetic and post-transcriptional silencing of transposable elements consequently protecting genomic integrity. HENMT1's activity is fundamental for safeguarding the genome from potential aberrations caused by active transposons.

Pathways

HENMT1 plays a significant part in the piRNA pathway which is important for gene silencing in the germline. Through this pathway it contributes to the protection of the genome against transposable elements by working alongside proteins like Piwi. The RNA interference (RNAi) pathway also involves HENMT1 although indirectly as RNAi and piRNA pathways share some mechanistic similarities in their mode of silencing unwanted genetic elements.

HENMT1 is connected to male infertility and certain cancers. Defects or malfunctions in HENMT1 can disrupt piRNA production leading to genomic instability and contributing to infertility in males. Additionally irregularities in its function might link it to cancer development possibly through faulty regulation of transposons. Other proteins like Piwi are relevant to understanding HENMT1's involvement in these conditions due to their collaborative function in the piRNA complex.
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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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제품 프로토콜

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Product promise

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