- Sanger seq
Unknown
Sanger Sequencing - Human HES4 knockout A549 cell lysate (AB258901)
Allele-1 : 46 bp deletion in exon4
- Sanger seq
Unknown
Sanger Sequencing - Human HES4 knockout A549 cell lysate (AB258901)
Allele-2 : 79 bp deletion in exon4
보완 제품
제품 세부 정보
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
Knockout 검증
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The function of HES4 involves transcriptional regulation of genes important for developmental processes. It often acts as a part of a transcriptional repression complex modulating gene expression patterns necessary for cellular differentiation. HES4 plays a vital role in the Notch signaling pathway influencing the balance between proliferation and differentiation of neural progenitor cells. By functioning within this complex HES4 ensures specific transcriptional outcomes are achieved impacting cell fate decisions.
Pathways
The involvement of HES4 in the Notch signaling pathway is significant. This pathway is pivotal for controlling cell fate and lineage commitment in various cell types particularly during nervous system development. Moreover HES4's interaction with other bHLH proteins such as HES1 further integrates its role into these pathways fine-tuning the outcome of cell differentiation processes. This interaction highlights the importance of HES4 in maintaining proper cellular function and development.
품질 관리
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com