JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB275250

Human HK2 (Hexokinase II) knockout HCT116 cell lysate

Be the first to review this product! 리뷰 제출

|

(0 출판물)

HK2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
2 이미지
Sanger Sequencing - Human HK2 (Hexokinase II) knockout HCT116 cell lysate (AB275250)
  • Sanger seq

Unknown

Sanger Sequencing - Human HK2 (Hexokinase II) knockout HCT116 cell lysate (AB275250)

Allele-1 : 1 bp insertion in exon 2.

Western blot - Human HK2 (Hexokinase II) knockout HCT116 cell lysate (AB275250)
  • WB

Lab

Western blot - Human HK2 (Hexokinase II) knockout HCT116 cell lysate (AB275250)

Lane 1 : Wild-type HCT116 cell lysate 20 ug
Lane 2 : HK2 knockout HCT116 cell lysate 20 ug
Lane 3 : HeLa cell lysate 20 ug
Lane 4 : SH-SY5Y cell lysate 20 ug
Lanes 1 - 4 : Merged signal (red and green). Green - ab209847 observed at 105 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab209847 was shown to react with Anti-Hexokinase II in wild-type HCT 116 cells in western blot with loss of signal observed in HK2 knockout cell line ab273721 (HK2 knockout cell lysate ab275250). HCT 116 wild-type and HK2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab209847 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Hexokinase II antibody [EPR20839] (<a href='/ko/products/primary-antibodies/hexokinase-ii-antibody-epr20839-ab209847'>ab209847</a>) at 1/1000 dilution

Lane 1:

Wild-type HCT116 cell lysate at 20 µg

Lane 2:

Western blot - Human HK2 (Hexokinase II) knockout HCT116 cell lysate (ab275250) at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 102 kDa

Observed band size: 105 kDa

false

주요 정보

세포 유형

HCT116

Species or organism

Human

조직

Colon

Knockout 검증

Sanger Sequencing,Western blot

Mutation 정보

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2

질병

Carcinoma

style
left-column

보완 제품

Assay Kits

AB207621

Human Heme Oxygenase 1 ELISA Kit

Recombinant|SimpleStep

ELISA - Human Heme Oxygenase 1 ELISA Kit (AB207621)

  • 0
  • 0
Assay Kits

AB176635

AKT1 + AKT2 + AKT3 (pS473) ELISA Kit

SimpleStep

ELISA - AKT1 + AKT2 + AKT3 (pS473) ELISA Kit (AB176635)

  • 0
  • 0
Assay Kits

AB269558

Human soluble EGFR ELISA Kit

Recombinant|SimpleStep

ELISA - Human soluble EGFR ELISA Kit (AB269558)

  • 0
  • 0
Assay Kits

AB176660

ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ELISA Kit

SimpleStep

ELISA - ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187) + Total ELISA Kit (AB176660)

  • 5
  • 4
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style
left-column

제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab275250-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human HK2 knockout HCT116 cell lysate", "number":"AB275250-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HCT116 cell lysate", "number":"AB275250-CMP02", "productcode":"" } ] } } }
style
left-column

특성 및 보관 정보

유전자명
HK2
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing, Western blot
Zygosity
Homozygous
품질 관리 - STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
style
left-column
style
left-column

Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

style
left-column

제품 프로토콜

style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com