- Sanger seq
Unknown
Sanger Sequencing - Human KLHDC3 knockout HEK-293T cell lysate (AB263242)
Homozygous : 1 bp insertion in exon2
보완 제품
제품 세부 정보
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
Knockout 검증
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
KLHDC3 participates in cellular processes such as transcriptional regulation and cell cycle control. It is involved in forming protein complexes that modify transcriptional responses. These complexes interact with a variety of transcription factors impacting gene expression. KLHDC3 also regulates the stability and degradation of certain proteins by acting as an adapter for ubiquitination processes.
Pathways
KLHDC3 plays a role in critical pathways like the ubiquitin-proteasome system and the Wnt signaling pathway. It interacts with proteins involved in these pathways such as Beta-catenin influencing cellular growth and development. The ubiquitin-proteasome system is essential for protein turnover while Wnt signaling regulates gene transcription that affects embryogenesis and cell proliferation.
품질 관리
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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