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AB258933

Human KMT2C (MLL3) knockout HeLa cell lysate

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KMT2C KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 239 bp insertion in exon6.
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Sanger Sequencing - Human KMT2C (MLL3) knockout HeLa cell lysate (AB258933)
  • Sanger seq

Unknown

Sanger Sequencing - Human KMT2C (MLL3) knockout HeLa cell lysate (AB258933)

Homozygous : 239 bp insertion in exon6

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, 239 bp insertion in exon6.

질병

Adenocarcinoma

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보완 제품

Primary Antibodies

AB26

Anti-p53 antibody [PAb 240]

KO Validated|Lab Essentials

Western blot - Anti-p53 antibody [PAb 240] (AB26)

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Primary Antibodies

AB231239

Anti-KMT2D / MLL2 antibody

Western blot - Anti-KMT2D / MLL2 antibody (AB231239)

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Primary Antibodies

AB108931

Anti-HAUSP / USP7 antibody [EPR4253]

RabMAb|Recombinant|KO Validated

Western blot - Anti-HAUSP / USP7 antibody [EPR4253] (AB108931)

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  • 2
Cell Lines & Lysates

AB265223

Human KMT2C (MLL3) knockout HeLa cell line

Sanger Sequencing - Human KMT2C (MLL3) knockout HeLa cell line (AB265223)

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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258933-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human KMT2C knockout HeLa cell lysate", "number":"AB258933-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB258933-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
KMT2C
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

KMT2C also known as MLL3 is a histone methyltransferase enzyme. Its mass is approximately 553 kDa. This protein exists in various tissues but expresses highly in the brain and bone marrow. It is part of the SET1 family of methyltransferases and plays an important role in modifying chromatin architecture by catalyzing the methylation of histone H3 lysine 4 (H3K4) a modification associated with active transcription.
Biological function summary

KMT2C impacts diverse cellular functions through its participation in the COMPASS-like complex which acetylates histone tails to regulate gene expression. This function is important in controlling genes linked to development and differentiation. KMT2C also supports the maintenance of genomic stability. Mutations in this protein often disrupt important brain and immune processes implicating its importance in physiological conditions.

Pathways

KMT2C plays a vital role in the Wnt and Hedgehog signaling pathways. These pathways regulate embryonic development cell fate decisions and cellular proliferation. Interaction with proteins such as β-catenin and GLI3 links KMT2C with the transcriptional activation of target genes further extending its influence on gene expression regulation and developmental processes.

Mutations or dysregulation of KMT2C are linked to cancers and Kabuki syndrome. In cancers KMT2C often exhibits loss-of-function mutations impacting tumor suppressor capabilities. In Kabuki syndrome alterations in KMT2C disrupt developmental signaling manifesting in intellectual disabilities and characteristic facial features. Interactions with proteins like EZH2 and KDM6A suggest KMT2C's critical involvement in disease pathogenesis and potential pathways for therapeutic intervention.
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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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제품 프로토콜

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Product promise

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