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AB263785

Human KRT8 (Cytokeratin 8) knockout HeLa cell lysate

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KRT8 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and 2 bp deletion in exon 2 and 4 bp deletion in exon 2.
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Sanger Sequencing - Human KRT8 (Cytokeratin 8) knockout HeLa cell lysate (AB263785)
  • Sanger seq

Unknown

Sanger Sequencing - Human KRT8 (Cytokeratin 8) knockout HeLa cell lysate (AB263785)

Allele-2 : 2 bp deletion in exon 2

Sanger Sequencing - Human KRT8 (Cytokeratin 8) knockout HeLa cell lysate (AB263785)
  • Sanger seq

Unknown

Sanger Sequencing - Human KRT8 (Cytokeratin 8) knockout HeLa cell lysate (AB263785)

Allele-3 : 1 bp insertion in exon 2

Sanger Sequencing - Human KRT8 (Cytokeratin 8) knockout HeLa cell lysate (AB263785)
  • Sanger seq

Unknown

Sanger Sequencing - Human KRT8 (Cytokeratin 8) knockout HeLa cell lysate (AB263785)

Allele-1 : 4 bp deletion in exon 2

Western blot - Human KRT8 (Cytokeratin 8) knockout HeLa cell lysate (AB263785)
  • WB

Lab

Western blot - Human KRT8 (Cytokeratin 8) knockout HeLa cell lysate (AB263785)

Lane 1 : A431 cell lysate (20 μg)

Lane 2 : MCF7 cell lysate (20 μg)

Lane 3 : Wild-type HeLa cell lysate (20 μg)

Lane 4 : KRT8 knockout HeLa cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab53280 observed at 55 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab53280 was shown to react with Cytokeratin 8 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255400 (knockout cell lysate ab263785) was used. Wild-type and Cytokeratin 8 knockout samples were subjected to SDS-PAGE. ab53280 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 (For unpurified use at 1/25,000 - 1/50,000) dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (<a href='/ko/products/primary-antibodies/cytokeratin-8-antibody-ep1628y-cytoskeleton-marker-ab53280'>ab53280</a>) at 1/10000 dilution

Lane 1:

A431 cell lysate at 20 µg

Lane 2:

MCF7 cell lysate at 20 µg

Lane 3:

Wild-type HeLa cell lysate at 20 µg

Lane 4:

Western blot - Human KRT8 (Cytokeratin 8) knockout HeLa cell lysate (ab263785) at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 53 kDa

Observed band size: 37 kDa,52 kDa

false

Western blot - Human KRT8 (Cytokeratin 8) knockout HeLa cell lysate (AB263785)
  • WB

Supplier Data

Western blot - Human KRT8 (Cytokeratin 8) knockout HeLa cell lysate (AB263785)

Lane 1 : A431 cell lysate (20 μg)

Lane 2 : MCF7 cell lysate (20 μg)

Lane 3 : Wild-type HeLa cell lysate (20 μg)

Lane 4 : KRT8 knockout HeLa cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab9023 observed at 55 kDa. Red - loading control, ab181602 observed at 37 kDa.

ab9023 was shown to react with Cytokeratin 8 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255400 (knockout cell lysate ab263785) was used. Wild-type and Cytokeratin 8 knockout samples were subjected to SDS-PAGE. ab9023 and Anti-GAPDH antibody EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Cytokeratin 8 antibody [M20] - Cytoskeleton Marker (<a href='/ko/products/primary-antibodies/cytokeratin-8-antibody-m20-cytoskeleton-marker-ab9023'>ab9023</a>) at 1/1000 dilution

Lane 1:

A431 cell lysate at 20 µg

Lane 2:

MCF7 cell lysate at 20 µg

Lane 3:

Wild-type HeLa cell lysate at 20 µg

Lane 4:

Western blot - Human KRT8 (Cytokeratin 8) knockout HeLa cell line (<a href='/ko/products/cell-lines/human-krt8-cytokeratin-8-knockout-hela-cell-line-ab255400'>ab255400</a>)

Lane 4:

Western blot - Human KRT8 (Cytokeratin 8) knockout HeLa cell lysate (ab263785)

Secondary

All lanes:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-800cw-preadsorbed-ab216772'>ab216772</a>) at 1/20000 dilution

Predicted band size: 53 kDa

Observed band size: 37 kDa

false

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

Knockout 검증

Sanger Sequencing,Western blot

Mutation 정보

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and 2 bp deletion in exon 2 and 4 bp deletion in exon 2.

질병

Adenocarcinoma

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보완 제품

Cell Lines & Lysates

AB255400

Human KRT8 (Cytokeratin 8) knockout HeLa cell line

Advanced Validation

Western blot - Human KRT8 (Cytokeratin 8) knockout HeLa cell line (AB255400)

  • 0
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Primary Antibodies

AB53280

Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (AB53280)

  • 5
  • 9
Proteins & Peptides

AB156970

Recombinant Human Cytokeratin 8 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human Cytokeratin 8 protein (His tag N-Terminus) (AB156970)

  • 0
  • 0
Proteins & Peptides

AB217407

Recombinant human Vitronectin/S-Protein (Animal Free)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab263785-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB263785-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human KRT8 knockout HeLa cell lysate", "number":"AB263785-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
KRT8
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing, Western blot
품질 관리 - STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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제품 프로토콜

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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