- Sanger seq
Unknown
Sanger Sequencing - Human MAN1C1 (HMIC) knockout HeLa cell lysate (AB258952)
Allele-1 : 28 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human MAN1C1 (HMIC) knockout HeLa cell lysate (AB258952)
Allele-2 : 1 bp deletion in exon3
보완 제품
제품 세부 정보
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
Knockout 검증
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
HMIC influences transcription regulation by participating in the modification of histone tails. It forms part of a larger multi-protein assembly that targets specific lysine residues on histones. This modification alters the chromatin's accessibility to transcription machinery impacting gene expression levels. By modulating histone acetylation and methylation states HMIC links closely with cellular processes like DNA replication and repair.
Pathways
HMIC functions within the chromatin remodeling pathways especially affecting the gene transcription pathway. It involves interactions with proteins such as transcription factors and other chromatin remodelers like Polycomb group proteins. These interactions enable the precise regulation of genes necessary for cell differentiation and development as well as in response to environmental signals.
품질 관리
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com