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AB257537

Human MELK knockout HCT116 cell lysate

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MELK KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon9 and Insertion of the selection cassette in exon9.

대체 명칭 보기

Tyrosine-protein kinase MELK, hMELK, Protein kinase PK38, pEg3 kinase, Protein kinase Eg3, MELK, hPK38, Maternal embryonic leucine zipper kinase, KIAA0175

3 이미지
Western blot - Human MELK knockout HCT116 cell lysate (AB257537)
  • WB

Lab

Western blot - Human MELK knockout HCT116 cell lysate (AB257537)

Lane 1 : Wild-type HCT116 cell lysate 20 ug
Lane 2 : MELK knockout HCT116 cell lysate 20 ug
Lanes 1 - 2 : Merged signal (red and green). Green - ab108529 observed at 75 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab108529 was shown to react with MELK in wild-type HCT116 cells in western blot with loss of signal observed in MELK knockout cell line ab266896 (MELK knockout cell lysate ab257537). Wild-type and MELK knockout HCT116 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108529 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MELK antibody [EPR3981] (<a href='/ko/products/primary-antibodies/melk-antibody-epr3981-ab108529'>ab108529</a>) at 1/1000 dilution

Lane 1:

HCT116 cell lysate at 20 µg

Lane 2:

MELK knockout HCT116 cell lysate at 20 µg

Lane 2:

Western blot - Human MELK knockout HCT116 cell line (<a href='/ko/products/cell-lines/human-melk-knockout-hct116-cell-line-ab266896'>ab266896</a>)

Predicted band size: 74 kDa

Observed band size: 75 kDa

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Sanger Sequencing - Human MELK knockout HCT116 cell lysate (AB257537)
  • Sanger seq

Unknown

Sanger Sequencing - Human MELK knockout HCT116 cell lysate (AB257537)

Allele-1 : 1 bp deletion in exon9

Sanger Sequencing - Human MELK knockout HCT116 cell lysate (AB257537)
  • Sanger seq

Unknown

Sanger Sequencing - Human MELK knockout HCT116 cell lysate (AB257537)

Allele-2 : Insertion of the selection cassette in exon9

주요 정보

세포 유형

HCT116

Species or organism

Human

조직

Colon

Knockout 검증

Sanger Sequencing,Western blot

Mutation 정보

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon9 and Insertion of the selection cassette in exon9.

질병

Carcinoma

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보완 제품

Primary Antibodies

AB108529

Anti-MELK antibody [EPR3981]

RabMAb|Recombinant|KO Validated

Western blot - Anti-MELK antibody [EPR3981] (AB108529)

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Proteins & Peptides

AB271456

Recombinant human CDK1 + Cyclin B1 protein (Active) (GST tag N-Terminus)

Functional Studies - Recombinant human CDK1 + Cyclin B1 protein (Active) (GST tag N-Terminus) (AB271456)

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Proteins & Peptides

AB204155

Recombinant human MELK protein (Active)

Functional Studies - Recombinant human MELK protein (Active) (AB204155)

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Cell Lines & Lysates

AB266896

Human MELK knockout HCT116 cell line

Advanced Validation

Western blot - Human MELK knockout HCT116 cell line (AB266896)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257537-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HCT116 cell lysate", "number":"AB257537-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human MELK knockout HCT116 cell lysate", "number":"AB257537-CMP01", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
MELK
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing, Western blot
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Maternal embryonic leucine zipper kinase commonly referred to as MELK is a serine/threonine protein kinase with a molecular mass of about 74 kDa. MELK is broadly expressed in both human and animal tissues with significant presence in embryonic and adult stem cells. Researchers often recognize it as MELK or MELK-0A21 in studies. The protein is characterized by its regulation of cell division and apoptosis making it an important player in cellular proliferation.
Biological function summary

MELK regulates signals that control the cell cycle apoptosis and embryonic development. MELK works as a component of complexes involved in maintaining cellular homeostasis. The protruding roles involve influencing cellular architecture and governance of stem cell maintenance. Studies in animal models show MELK involvement in maintaining pluripotency and regulation during the development phase.

Pathways

MELK participates in key processes such as the cell cycle and apoptosis pathways. Its diverse functionality connects MELK to proteins like Bcl-2-associated death promoter (BAD) and p53 which further influence main cellular events. These pathways and interactions make MELK a target of interest in the study of cellular growth and programmed cell death.

Researchers link MELK to cancer and neurodegenerative diseases. The overexpression of MELK is frequently observed in various cancer types making it relevant to oncogenesis. Studies examine its relation to proteins like cyclin-dependent kinases (CDKs) and survivin in cancer progression. Additionally MELK-0A21 variants may offer insights in disorders like Glioblastoma where abnormal MELK activity aligns with disease aggression.
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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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제품 프로토콜

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Product promise

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