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AB258050

Human MPLKIP (TTDN1) knockout HeLa cell lysate

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MPLKIP KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.

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TTDN1, MPLKIP, C7orf11, TTD non-photosensitive 1 protein, M-phase-specific PLK1-interacting protein

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Sanger Sequencing - Human MPLKIP (TTDN1) knockout HeLa cell lysate (AB258050)
  • Sanger seq

Unknown

Sanger Sequencing - Human MPLKIP (TTDN1) knockout HeLa cell lysate (AB258050)

Allele-2 : 1 bp deletion in exon 1

Sanger Sequencing - Human MPLKIP (TTDN1) knockout HeLa cell lysate (AB258050)
  • Sanger seq

Unknown

Sanger Sequencing - Human MPLKIP (TTDN1) knockout HeLa cell lysate (AB258050)

Allele-1 : Insertion of the selection cassette in exon 1

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.

질병

Adenocarcinoma

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보완 제품

Primary Antibodies

AB76256

Anti-eIF4E (phospho S209) antibody [EP2151Y]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-eIF4E (phospho S209) antibody [EP2151Y] (AB76256)

  • 5
  • 2
Primary Antibodies

AB246518

Anti-CXCL13 antibody [EPR23400-92]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL13 antibody [EPR23400-92] (AB246518)

  • 5
  • 1
Cell Lines & Lysates

AB258713

Human TAP2 knockout A549 cell lysate

Sanger Sequencing - Human TAP2 knockout A549 cell lysate (AB258713)

  • 0
  • 0
Primary Antibodies

AB133327

Anti-CDK1 antibody [EPR165]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CDK1 antibody [EPR165] (AB133327)

  • 5
  • 4
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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258050-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB258050-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human MPLKIP knockout HeLa cell lysate", "number":"AB258050-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
MPLKIP
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

TTDN1 also known as C20orf174 is a protein involved in cellular processes that facilitate DNA repair. It weighs approximately 47 kilodaltons and is expressed in various tissues with higher levels observed in testis and ovary. The protein plays an essential role in maintaining genomic integrity by interacting with specific components of the DNA repair machinery.
Biological function summary

TTDN1 contributes to the repair of double-strand DNA breaks. It forms part of the larger DNA repair complex which recruits and coordinates other proteins needed for effective DNA repair. This function is especially important in cells exposed to frequent DNA damage supporting cellular stability and proper functioning by ensuring accurate DNA replication and transmission.

Pathways

The functions of TTDN1 intersect with the homologous recombination and non-homologous end joining pathways. It interacts with proteins such as BRCA1 and RAD51 which are key in DNA damage response and repair. These pathways enable cells to prevent mutations therefore safeguarding the genome's integrity during cell division and proliferation.

TTDN1 is associated with trichothiodystrophy a condition characterized by defects in DNA repair mechanisms. This association suggests TTDN1's involvement in clinical presentations linked to DNA repair deficiencies. Additionally anomalies in proteins like ERCC2 which also participates in DNA repair may exacerbate the effects when TTDN1 function is impaired leading to more severe disease outcomes.
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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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제품 프로토콜

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Product promise

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