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AB258984

Human NACA (NACA1) knockout HEK-293T cell lysate

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NACA KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2.
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Sanger Sequencing - Human NACA (NACA1) knockout HEK-293T cell lysate (AB258984)
  • Sanger seq

Unknown

Sanger Sequencing - Human NACA (NACA1) knockout HEK-293T cell lysate (AB258984)

Homozygous : Insertion of the selection cassette in exon 2

주요 정보

세포 유형

HEK-293T

Species or organism

Human

조직

Kidney

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2.

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보완 제품

Primary Antibodies

AB221950

Anti-NACA1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NACA1 antibody (AB221950)

  • 4
  • 2
Assay Kits

AB171577

Human HIF-1 alpha ELISA Kit

SimpleStep

ELISA - Human HIF-1 alpha ELISA Kit (AB171577)

  • 3
  • 1
Primary Antibodies

AB133497

Anti-Flotillin 1 antibody [EPR6041]

RabMAb|Recombinant|KO Validated|20ul selling size

Flow Cytometry (Intracellular) - Anti-Flotillin 1 antibody [EPR6041] (AB133497)

  • 5
  • 6
Assay Kits

AB133023

6-keto-PGF1 alpha ELISA Kit

Competitive Binding Assays - 6-keto-PGF1 alpha ELISA Kit (AB133023)

  • 0
  • 0
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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258984-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB258984-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human NACA knockout HEK293T cell lysate", "number":"AB258984-CMP01", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
NACA
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing
Zygosity
Homozygous
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

NACA1 also known as Nascent Polypeptide-Associated Complex Alpha-1 is a vital protein in cells with a molecular mass of around 21 kDa. It plays a big role as a transcription factor and coordinates the synthesis and proper folding of nascent polypeptides. This protein shows high expression in various tissues including muscle brain and liver. As a multifunctional player NACA1 assists in safeguarding polypeptides emerging during protein synthesis ensuring they achieve correct conformation and function.
Biological function summary

NACA1 modulates transcriptional activity interacting with particular sequences in DNA to affect gene expression. It forms a part of the nascent polypeptide-associated complex (NAC) that helps modulate developing polypeptides and ribosome-associated processes. By working in tandem with other proteins NACA1 not only contributes to protein folding but also aids in the regulation of stress responses. This highlights its importance in ensuring proteins function correctly under various cellular conditions.

Pathways

NACA1 engages in the regulation of protein homeostasis and gene expression pathways. It is involved in the unfolded protein response (UPR) and interacts with other ribosome-associated proteins. NACA1 plays a role in stress-response pathways ensuring proteins are correctly processed and folded under stress conditions. It may interact with other proteins like Hsp70 and ribosomal proteins to enhance protein quality control ensuring normal cellular function.

NACA1's dysregulation relates to conditions such as myopathies and cancer. Altered expression or function can disrupt normal stress response pathways resulting in muscle weakness or tumor progression. Researchers have noted its potential link with BRCA1-associated breast cancer and impacts seen in muscular diseases suggesting it may influence disease progression through its interaction with genes and proteins central to these disorders. Understanding NACA1’s role can support the development of new therapeutic strategies for these conditions.
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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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제품 프로토콜

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Product promise

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