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AB259039

Human PHIP knockout HeLa cell lysate

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PHIP KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.

대체 명칭 보기

PHIP, IRS-1 PH domain-binding protein, PH-interacting protein, DDB1- and CUL4-associated factor 14, DCAF14, WDR11, WD repeat-containing protein 11

3 이미지
Sanger Sequencing - Human PHIP knockout HeLa cell lysate (AB259039)
  • Sanger seq

Unknown

Sanger Sequencing - Human PHIP knockout HeLa cell lysate (AB259039)

Allele-2 : 1 bp insertion in exon1

Sanger Sequencing - Human PHIP knockout HeLa cell lysate (AB259039)
  • Sanger seq

Unknown

Sanger Sequencing - Human PHIP knockout HeLa cell lysate (AB259039)

Allele-1 : 1 bp deletion in exon1

Western blot - Human PHIP knockout HeLa cell lysate (AB259039)
  • WB

Supplier Data

Western blot - Human PHIP knockout HeLa cell lysate (AB259039)

All lanes:

Western blot - Anti-PHIP antibody [EPR27150-82] (<a href='/ko/products/primary-antibodies/phip-antibody-epr27150-82-ab309962'>ab309962</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa(human cervical adenocarcinoma epithelial cell) whole cell lysate at 40 µg

Lane 2:

Western blot - Human PHIP knockout HeLa cell lysate (ab259039) at 40 µg

Lane 3:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 250 kDa

false

Exposure time: 3min

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.

질병

Adenocarcinoma

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보완 제품

Primary Antibodies

AB52649

Anti-GM130 antibody [EP892Y] - cis-Golgi Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunoprecipitation - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (AB52649)

  • 5
  • 39
Primary Antibodies

AB109110

Anti-EEA1 antibody [EPR4245] - Early Endosome Marker

20ul selling size|RabMAb|Recombinant|KO Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-EEA1 antibody [EPR4245] - Early Endosome Marker (AB109110)

  • 5
  • 4
Primary Antibodies

AB133497

Anti-Flotillin 1 antibody [EPR6041]

RabMAb|Recombinant|KO Validated|20ul selling size

Flow Cytometry (Intracellular) - Anti-Flotillin 1 antibody [EPR6041] (AB133497)

  • 5
  • 6
Primary Antibodies

AB52599

Anti-Histone H2B antibody [EP957Y]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Histone H2B antibody [EP957Y] (AB52599)

  • 4
  • 4
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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab259039-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB259039-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human PHIP knockout HeLa cell lysate", "number":"AB259039-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
PHIP
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PHIP also known as pleckstrin homology domain interacting protein is an adapter protein that interacts with insulin receptor substrates. It has a mass of approximately 100 kDa. PHIP is expressed in various tissues including the brain and liver. The protein is involved in intracellular signaling pathways by binding phosphoinositides which are important for cellular communication and growth regulation.
Biological function summary

PHIP plays a significant role in cell proliferation and survival. It contributes to the regulation of the cell cycle by influencing transcription factors and other core components involved in cell growth. PHIP is not only a standalone protein but also part of complexes that modulate chromatin affecting gene expression patterns.

Pathways

PHIP is integrated into insulin signaling and PI3K/AKT pathways. In these pathways PHIP interacts with key proteins like AKT1 supporting cellular responses to insulin and growth factors. The involvement of PHIP in these pathways highlights its role in metabolic regulation and its impact on cell growth and metabolism.

PHIP has links to cancer and diabetes. Its dysregulation contributes to abnormal cell proliferation often observed in cancers. PHIP's interaction with insulin signaling components such as IRS1 demonstrates its relevance to diabetes management. These connections show PHIP's potential as a therapeutic target for these conditions.
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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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제품 프로토콜

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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