- Sanger seq
Unknown
Sanger Sequencing - Human PIP4K2A knockout HeLa cell lysate (AB259041)
Allele-1 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human PIP4K2A knockout HeLa cell lysate (AB259041)
Allele-2 : Insertion of the selection cassette in exon1
보완 제품
제품 세부 정보
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
제품 구성
특성 및 보관 정보
유전자명
유전자 편집 종류
유전자 편집 방식
Knockout 검증
배송 시 보관 조건
적절한 단기 보관 조건
적절한 장기 보관 조건
추가 정보
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PIP4K2 alpha participates in the regulation of phosphoinositide signaling and lipid metabolism. It contributes to the modulation of various cellular processes including cell growth and survival. PIP4K2 alpha doesn't form a large complex but interacts with other kinases to influence signaling pathways. This protein acts as a regulator of PI(45)P2 levels balancing phosphatidylinositol lipid signaling in cells.
Pathways
PIP4K2 alpha plays important roles in the phosphatidylinositol signaling pathway and the insulin signaling pathway. In the phosphatidylinositol signaling pathway PIP4K2 alpha works alongside other kinases like PIP5K1A to regulate the synthesis of PI(45)P2. In the insulin signaling pathway PIP4K2 alpha contributes to the regulation of glucose uptake and metabolism maintaining cellular energy balance.
품질 관리
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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