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AB259042

Human PITPNB knockout HeLa cell lysate

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PITPNB KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.

대체 명칭 보기

PI-TP-beta, PtdInsTP beta, Phosphatidylinositol transfer protein beta isoform, PITPNB, PtdIns transfer protein beta

2 이미지
Sanger Sequencing - Human PITPNB knockout HeLa cell lysate (AB259042)
  • Sanger seq

Unknown

Sanger Sequencing - Human PITPNB knockout HeLa cell lysate (AB259042)

Allele-2 : Insertion of the selection cassette in exon 2

Sanger Sequencing - Human PITPNB knockout HeLa cell lysate (AB259042)
  • Sanger seq

Unknown

Sanger Sequencing - Human PITPNB knockout HeLa cell lysate (AB259042)

Allele-1 : 1 bp deletion in exon 2

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

Knockout 검증

Sanger Sequencing

Mutation 정보

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.

질병

Adenoma

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보완 제품

Primary Antibodies

AB259806

Anti-CD127 antibody [EPR23747-333]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD127 antibody [EPR23747-333] (AB259806)

  • 4
  • 1
Primary Antibodies

AB302922

Anti-IL-17A antibody [EPR26410-51]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-IL-17A antibody [EPR26410-51] (AB302922)

  • 0
  • 0
Primary Antibodies

AB133543

Anti-Liver Arginase antibody [EPR6672(B)]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Liver Arginase antibody [EPR6672(B)] (AB133543)

  • 5
  • 3
Primary Antibodies

AB313962

PE Anti-IL-21R antibody [EPR23637-53]

RabMAb|Recombinant

Flow Cytometry - PE Anti-IL-21R antibody [EPR23637-53] (AB313962)

  • 0
  • 0
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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab259042-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB259042-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human PITPNB knockout HeLa cell lysate", "number":"AB259042-CMP02", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
PITPNB
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

PITPNB also known as phosphatidylinositol transfer protein beta isoform plays a role mechanically in lipid transfer between membranes. It weighs around 32 kDa. You can find this protein expressed in various tissues including the brain liver and lungs. PITPNB facilitates lipid metabolism and signaling by transferring phosphatidylinositol and phosphatidylcholine between membranes.
Biological function summary

PITPNB ensures the proper distribution of lipids within cells for membrane composition and signaling activities. It participates in maintaining the balance of lipid composition among cell membranes. PITPNB does not function alone; it often acts as part of lipid transfer complexes collaborating with other proteins to execute its function properly.

Pathways

PITPNB plays an active role in phosphoinositide signaling and membrane trafficking pathways. These pathways are critical for cell signaling and membrane dynamics. PI4K2A and PI4KA are proteins related to these pathways and interact with PITPNB highlighting its contribution to complex intracellular signaling processes.

Defects in PITPNB have been linked to metabolic disorders and neurodegenerative diseases such as Alzheimer's. PITPNB is connected to APOE a protein involved in lipid metabolism and associated with Alzheimer's disease progression. Understanding the role of PITPNB in these conditions opens possibilities for therapeutic strategies targeting lipid transfer processes.
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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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제품 프로토콜

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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