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AB258587

Human PKN2 knockout HeLa cell lysate

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PKN2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 5.
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Western blot - Human PKN2 knockout HeLa cell lysate (AB258587)
  • WB

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Western blot - Human PKN2 knockout HeLa cell lysate (AB258587)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : PKN2 knockout HeLa cell lysate (20 μg)

Lane 3 : 293T cell lysate (20 μg)

Lanes 1-3 : Merged signal (red and green). Green - ab138514 observed at 112 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab138514 Anti-PKN2 antibody [EPR5490] was shown to specifically react with PKN2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264691 (knockout cell lysate ab258587) was used. Wild-type and PKN2 knockout samples were subjected to SDS-PAGE. ab138514 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PKN2 antibody [EPR5490] (<a href='/ko/products/primary-antibodies/pkn2-antibody-epr5490-ab138514'>ab138514</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PKN2 knockout HeLa cell lysate at 20 µg

Lane 3:

293T cell lysate at 20 µg

Predicted band size: 112 kDa

Observed band size: 112 kDa

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Sanger Sequencing - Human PKN2 knockout HeLa cell lysate (AB258587)
  • Sanger seq

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Sanger Sequencing - Human PKN2 knockout HeLa cell lysate (AB258587)

Homozygous : 1 bp insertion in exon 5

주요 정보

세포 유형

HeLa

Species or organism

Human

조직

Cervix

Knockout 검증

Sanger Sequencing,Western blot

Mutation 정보

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 5.

질병

Adenocarcinoma

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보완 제품

Secondary Antibodies

AB207995

Goat Anti-Rabbit IgG H&L (Biotin)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (AB207995)

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Primary Antibodies

AB248734

Anti-Dynamin 3 antibody [EPR9055] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Dynamin 3 antibody [EPR9055] - BSA and Azide free (AB248734)

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Primary Antibodies

AB125938

Rabbit IgG, monoclonal [SP137] - isotype control

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG, monoclonal [SP137] - isotype control (AB125938)

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Secondary Antibodies

AB207996

Goat Anti-Mouse IgG H&L (Biotin)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (Biotin) (AB207996)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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제품 세부 정보

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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제품 구성

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258587-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB258587-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human PKN2 knockout HeLa cell lysate", "number":"AB258587-CMP01", "productcode":"" } ] } } }
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특성 및 보관 정보

유전자명
PKN2
유전자 편집 종류
Knockout
유전자 편집 방식
CRISPR technology
Knockout 검증
Sanger Sequencing, Western blot
Zygosity
Homozygous
배송 시 보관 조건
Ambient - Can Ship with Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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품질 관리

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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제품 프로토콜

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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